LITAF Antibody (1F9) - BSA Free

Western Blot: LITAF Antibody (1F9) [NBP1-30250] - The Cell lysates (40ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-humanLITAF antibody (1:1000). Proteins were visualized using agoat anti-mouse secondary antibody conjugated to HRP andan ECL detection system.Lane 1.: HeLa cell lysateLane 2.: HepG2 cell lysate

Immunocytochemistry/Immunofluorescence: LITAF Antibody (1F9) [NBP1-30250] - Analysis of LITAF in LNCap cells line, stained withDAPI (Blue) for nucleus staining and monoclonal anti-humanLITAF antibody (1:100) with goat anti-mouse IgG-Alexa fluor488 conjugate (Green).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

LITAF Antibody (1F9) - BSA Free Summary

• Immunogen: Recombinant human LITAF (1-161aa) purified from E. coli
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LITAF
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for LITAF Antibody (1F9) - BSA Free

• CMT1C
• FLJ38636
• lipopolysaccharide-induced TNF factor
• lipopolysaccharide-induced TNF-alpha factor
• lipopolysaccharide-induced tumor necrosis factor-alpha factor
• LITAF
• LPS-induced TNF-alpha factor
• MGC116698
• MGC116700
• MGC125275
• MGC125276
• p53-induced gene 7 protein
• PIG7
• PIG7MGC116701
• SIMPLE
• SIMPLEMGC125274
• Small integral membrane protein of lysosome/late endosome
• TP53I7
• tumor protein p53 inducible protein 7

Background

Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppresor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget's disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LITAF
• Entrez:
  • 9516(Human)
• Uniprot:
  • NP_004853(Human)