Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free

Western Blot: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Analysis in human cell lines A-431 and U-251MG using anti-LSR antibody. Corresponding LSR RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.

Immunocytochemistry/Immunofluorescence: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human endometrium shows strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human kidney shows weak positivity in cells in tubules.

Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human liver shows moderate membranous positivity in hepatocytes.

Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human stomach shows strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human testis shows moderate membranous and cytoplasmic positivity in cells in seminiferous ducts.

Staining of human stomach shows strong membranous positivity in glandular cells.

Staining of human liver shows moderate membranous positivity in hepatocytes.

Simple Western: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Simple Western lane view shows a specific band for LSR in 0.2 mg/ml of RT-4 (left), A431 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Electropherogram image(s) of corresponding Simple Western lane view. Lipolysis Stimulated Lipoprotein Receptor antibody was used at 1:20 dilution on RT-4 and A431 lysate(s).

• Reactivity: Hu, Mu, Po, RM
• Applications: WB, Simple Western, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: MSEVTSLHEDDWRSRPSRGPALTPIRDEEWGGHSPRSPRGWDQEPAREQAGGGWRARRPRARSVDALDDLTPPSTAESGSRSPTSNGGRSRAYMPPRSRSRDDLYDQDDS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LSR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated Reported in scientific publication (PMID: 32423802).
  • Simple Western 1:20
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
  
  See Simple Western Antibody Database for Simple Western validation: only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue
• Control Peptide:

  Lipolysis Stimulated Lipoprotein Receptor Protein (NBP1-89631PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25753034). Primate (Rhesus macaques) reported in scientific literature (PMID: 26067369). Porcine reactivity reported in scientific literature (PMID: 24048008)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free

• ILDR3
• immunoglobulin-like domain containing receptor 3
• lipolysis stimulated lipoprotein receptor
• lipolysis-stimulated lipoprotein receptor
• lipolysis-stimulated receptor
• lipolysis-stimulated remnant
• LISCH protein
• LISCH
• LISCH7
• liver-specific bHLH-Zip transcription factor
• LSR
• MGC10659
• MGC48312
• MGC48503

Background

Probable role in the clearance of triglyceride-rich lipoprotein from blood. Binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lipolysis Stimulated Lipoprotein Receptor Antibody (NBP1-89631)(14)

Publications using NBP1-89631

• Arai W, Konno T, Kohno T et al. Downregulation of angulin-1/LSR induces malignancy via upregulation of EGF-dependent claudin-2 and TGF-?-dependent cell metabolism in human lung adenocarcinoma A549 cells Oncotarget 2023-03-24 [PMID: 36961882]
• Ohwada K, Konno T, Kohno T Et al. Effects of HMGB1 on Tricellular Tight Junctions via TGF-beta Signaling in Human Nasal Epithelial Cells International journal of molecular sciences 2021-08-04 [PMID: 34445093]
• Kohno T, Konno T, Kikuchi S Et al. Translocation of LSR from tricellular corners causes macropinocytosis at cell-cell interface as a trigger for breaking out of contact inhibition FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-09-01 [PMID: 34403506] (WB)
• Kakiuchi A, Kakuki T, Ohwada K et al. HDAC inhibitors suppress the proliferation, migration and invasiveness of human head and neck squamous cell carcinoma cells via p63?mediated tight junction molecules and p21?mediated growth arrest Oncology Reports 2021-03-01 [PMID: 33649777]
• Kodera Y, Kohno T, Konno T et al. HMGB1 enhances epithelial permeability via p63/TGF-beta signaling in lung and terminal bronchial epithelial cells Tissue Barriers 2020-08-28 [PMID: 32857676]
• Kodera Y, Chiba H, Konno T et al. HMGB1-downregulated angulin-1/LSR induces epithelial barrier disruption via claudin-2 and cellular metabolism via AMPK in airway epithelial Calu-3 cells Biochem. Biophys. Res. Commun. 2020-06-25 [PMID: 32423802] (ICC/IF, KD, WB, Human)
• Konno T, Kohno T, Kikuchi S et al. Epithelial barrier dysfunction and cell migration induction via JNK/cofilin/actin by angubindin-1 Tissue Barriers 2019-11-29 [PMID: 31782346]
• Konno T, Kohno T, Kikuchi S et al. Localization of Tricellular Tight Junction Molecule LSR at Midbody and Centrosome During Cytokinesis in Human Epithelial Cells J. Histochem. Cytochem. 2019-10-29 [PMID: 31662022]
• Konno T, Takano K, Kaneko Y et al. Guanylate binding protein-1-mediated epithelial barrier in human salivary gland duct epithelium. Exp. Cell Res. 2018-07-22 [PMID: 30044945] (IHC-P, Human)
• SHIMADA H, SATOHISA S, kOHNO T et al. Downregulation of lipolysis‑stimulated lipoprotein receptor promotes cell invasion via claudin‑1‑mediated matrix metalloproteinases in human endometrial cancer. Oncology letters. [PMID: 29151917] (WB, Human)
• Takano K, Kakuki T, Kaneko Y et al. Histone deacetylase inhibition prevents cell death induced by loss of tricellular tight junction proteins in temperature-sensitive mouse cochlear cells PLoS ONE 2017-08-02 [PMID: 28767685] (WB, Mouse)
• Miyata R, Kakuki T, Nomura K et al. Poly(I:C) induced microRNA-146a regulates epithelial barrier and secretion of proinflammatory cytokines in human nasal epithelial cells. Eur. J. Pharmacol. 2015-05-07 [PMID: 25959385] (WB, Human)
• Sohet F, Lin C, Munji RN et al. LSR/angulin-1 is a tricellular tight junction protein involved in blood-brain barrier formation. J Cell Biol 2015-03-16 [PMID: 25753034] (ICC/IF, Mouse)
• Reaves DK, Fagan-Solis KD, Dunphy K et al. The Role of Lipolysis Stimulated Lipoprotein Receptor in Breast Cancer and Directing Breast Cancer Cell Behavior. PLoS One 2014-01-01 [PMID: 24637461]

FAQs for Lipolysis Stimulated Lipoprotein Receptor Antibody (NBP1-89631). (Showing 1 - 1 of 1 FAQs).

1. In the datasheet of your antibody against the Lipolysis-stimulated lipoprotein receptor (NBP1-89631) you show a figure in which a immunohistochemistry staining is shown. Can you please give an explanation why the cytoplasm of the cells is stained? Because
   • Lipolysis Stimulated Lipoprotein Receptor/LSR is a 649 amino acids long (71.4 kDa) trans-membrane protein with Extracellular (aa 1-259), Helical (260-280) and Cytoplasmic (281 – 649) domains Uniprot Site . Please note that NBP1-89631 was generated against a peptide corresponding to aa 431-540 of human LSR, and this sequence falls within cytoplasmic domain of LSR protein. Accordingly, NBP1-89631 is expected to bind the cytoplasmic domain of LSR protein and in immunostaining assays, the expected staining pattern with this antibody would be cytoplasmic as shown in the validation image of its datasheet.

Bioinformatics

• Gene Symbol: LSR