Lipocalin-2/NGAL Antibody (5G5) - BSA Free

ELISA: Lipocalin-2/NGAL Antibody (5G5) - BSA Free [NBP2-23529] - The calibration curve of a sandwich assay for monomer human NGAL using NBP2-23529 as the capture antibody and NBP2-41365B as the biotinylated detection antibody(1).

Product Discontinued

Lipocalin-2/NGAL Antibody (5G5) - BSA Free Summary

• Immunogen: Human recombinant NGAL in monomer and dimer forms adsorbed to aluminum hydroxide gel
• Epitope: Epitope specificity differs from that Clone 02 and the series of antibodies.
• Specificity: Clone 5G5 is specific for human NGAL. No cross-reaction with NGAL from other species has been detected.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LCN2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Clone 5G5 reacts strongly with human NGAL. Strong reaction is seen when used as detection antibody in sandwich ELISA in combination with a polyclonal NGAL capture antibody and when tested on recombinant NGAL coated directly onto the well. It can also be sued as capture antibody in sandwich ELISA with biotinylated Clone 02 as detection antibody. Clone 01 reacts with NGAL in immunochemical staining of paraformaldehyde-fixed sections. In Western blotting of SDS-PAGE gels, Clone 5G5 reacts with NGAL in both reduced and unreduced forms

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Lipocalin-2/NGAL Antibody (5G5) - BSA Free

• 24p3
• 25 kDa alpha-2-microglobulin-related subunit of MMP-9
• HNL
• LCN2
• lipocalin 2 (oncogene 24p3)
• lipocalin 2
• Lipocalin2
• Lipocalin-2
• migration-stimulating factor inhibitor
• MSFI
• neutrophil gelatinase-associated lipocalin
• NGAL
• NGALlipocalin-2
• Oncogene 24p3
• p25
• Siderocalin
• Uterocalin

Background

Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B) . Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers . Expression of NGAL in the kidney is dramatically increased by acute kidney injury

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LCN2