Lipocalin-2/NGAL Antibody (220322R) [Unconjugated]

• Reactivity: Hu
• Applications: ELISA(Cap)
• Clone: 220322R
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Lipocalin-2/NGAL Antibody (220322R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Lipocalin-2/NGAL
  Gln21-Gly198
  Accession # P80188
• Specificity: Detects human Lipocalin-2/NGAL in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipocalin-2/NGAL Antibody (220322R) [Unconjugated]

• 24p3
• 25 kDa alpha-2-microglobulin-related subunit of MMP-9
• HNL
• LCN2
• lipocalin 2 (oncogene 24p3)
• lipocalin 2
• Lipocalin2
• Lipocalin-2
• migration-stimulating factor inhibitor
• MSFI
• neutrophil gelatinase-associated lipocalin
• NGAL
• NGALlipocalin-2
• Oncogene 24p3
• p25
• Siderocalin
• Uterocalin

Background

Members of Lipocalin family share a highly conserved fold with an eight-stranded antiparallel beta barrel, and act as a transporters, carrying small molecules to specific cells (1). Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules (2). It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9) (2). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation (3). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3 - 5). Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (K_d = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less K_d values (7.9 nM) (6). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin‑2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).

1. Flower, D.R. et al. (1994) FEBS Lett. 354:7.
2. Kjeldsen, L. et al. (1993) J. Biol. Chem. 268:10426.
3. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
4. Devireddy, L.R. et al. (2001) Science 293: 829.
5. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
6. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
7. Flo, T.H. et al. (2004) Nature 432:917.
8. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lipocalin-2/NGAL Antibody (MAB17571R)(1)

Publication using MAB17571R

• PE Thiriet, D Medagoda, G Porro, C Guiducci Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads Biosensors, 2020;10(12):. 2020-01-01 [PMID: 33371213] (ELISA Detection, Human)

Bioinformatics

• Uniprot:
  • P80188()