Lipin 1 Antibody (JG37-69)

Western Blot: Lipin 1 Antibody (JG37-69) [NBP2-76845] - Western blot analysis of Lipin 1 on different cell lysates using anti-Lipin 1 at 1/500 dilution.Positive control:Line 1: K562 Line 2: SH-SY-5YLine 3: Mouse lung

Immunocytochemistry/Immunofluorescence: Lipin 1 Antibody (JG37-69) [NBP2-76845] - ICC staining Lipin 1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Lipin 1 Antibody (JG37-69) [NBP2-76845] - Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using anti-Lipin 1 antibody. Counter stained with hematoxylin.

Flow Cytometry: Lipin 1 Antibody (JG37-69) [NBP2-76845] - Flow cytometric analysis of PC-3M cells with Lipin 1 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Lipin 1 Antibody (JG37-69) [NBP2-76845] - ICC staining Lipin 1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Lipin 1 Antibody (JG37-69) [NBP2-76845] - Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Lipin 1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: Lipin 1 Antibody (JG37-69) [NBP2-76845] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Lipin 1 antibody. Counter stained with hematoxylin.

Product Discontinued

Lipin 1 Antibody (JG37-69) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Lipin 1 aa 81-220 / 890. (SwissProt: Q14693 Human)
• Localization: Nucleus. Cytoplasm. Endoplasmic reticulum.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LPIN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000
• Theoretical MW: 98 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Lipin 1 Antibody (JG37-69)

• DKFZp781P1796
• EC 3.1.3.18
• EC 3.1.3.4
• EC 5.4.2.7
• KIAA0188phosphatidate phosphatase LPIN1
• Lipin 1
• lipin-1
• LPIN1
• PAP1

Background

LPIN1 (Lipin-1) is crucial for normal adipose tissue development and metabolism. LPIN1 selectively activates a subset of PGC-225; target pathways, including fatty acid oxidation and mitochondrial oxidative phosphorylation by inducing expression of the nuclear receptor PPAR 225. LPIN1 also inactivates the lipogenic program and suppresses circulating lipid levels. An abundance of LPIN1 promotes fat accumulation and insulin sensitivity. A deficiency in LPIN1 may deter normal adipose tissue development, resulting in insulin resistance and lipodystrophy, a heterogeneous group of disorders characterized by loss of body fat, fatty liver, hypertriglyceridemia and insulin resistance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LPIN1