LINGO-1 Antibody (332237)

CHP‑100 human neuro­blastoma cell line was stained with Mouse Anti-Human LINGO‑1 Mono­clonal Antibody (Catalog # MAB30861, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 332237
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

LINGO-1 Antibody (332237) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LINGO-1
  Thr40-Thr556
  Accession # NP_116197
• Specificity: Detects human LINGO-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) LINGO-2 is observed and no cross-reactivity with rhLINGO-3 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LINGO1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LINGO-1 Antibody (332237)

• FLJ14594
• LERN1
• LERN1UNQ201
• leucine rich repeat and Ig domain containing 1
• leucine rich repeat neuronal 6A
• Leucine-rich repeat and immunoglobilin domain-containing protein 1
• Leucine-rich repeat neuronal protein 1
• Leucine-rich repeat neuronal protein 6A
• LINGO1
• LINGO-1
• LRRN6A
• LRRN6Aleucine-rich repeat neuronal 6A
• MGC17422

Background

LINGO-1 (LRRN6A) is a 614 amino acid (aa) transmembrane protein of the leucine-rich repeat (LRR) family, ribonuclease inhibitor subfamily. The four known LINGO proteins contain LRR and IgCAM domains in the extracellular portion and share 44‑61% aa sequence identity. LINGO-1 is restricted to the nervous system and is concentrated in the brain as a component of the NgR1/p75 and NgR1/Taj (TROY) signaling complexes. LINGO-1 negatively regulates neurite outgrowth and myelination. LINGO-1 is highly conserved, showing 99% aa sequence identity between human, mouse and rat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LINGO-1 Antibody (MAB30861)(2)

Publications using MAB30861

• Anthony Guillemain, Yousra Laouarem, Laetitia Cobret, Dora Štefok, Wanyin Chen, Solal Bloch et al. LINGO family receptors are differentially expressed in the mouse brain and form native multimeric complexes The FASEB Journal 2020-10-01 [PMID: 32862444]
• Meabon J, De Laat R, Ieguchi K, Wiley J, Hudson M, Bothwell M LINGO-1 protein interacts with the p75 neurotrophin receptor in intracellular membrane compartments. J Biol Chem, 2015-02-09;290(15):9511-20. 2015-02-09 [PMID: 25666623] (IHC, Mouse)

Bioinformatics

• Gene Symbol: LINGO1
• Uniprot:
  • NP_116197()