Recombinant Human LIN-28B Protein

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SDS-Page: Lin28B Protein [NBP2-23196]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

Order Details

Recombinant Human LIN-28B Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-250 of Human LIN-28B

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEGGAS KGGGEEPGKL PEPAEEESQV LRGTGHCKWF NVRMGFGFIS MINREGSPLD IPVDVFVHQS KLFMEGFRSL KEGEPVEFTF KKSSKGLESI RVTGPGGSPC LGSERRPKGK TLQKRKPKGD RCYNCGGLDH HAKECSLPPQ PKKCHYCQSI MHMVANCPHK NVAQPPASSQ GRQEAESQPC TSTLPREVGG GHGCTSPPFP QEARAEISER SGRSPQEASS TKSSIAPEEQ SKKGPSVQKR KKT

Protein/Peptide Type
Recombinant Protein
Gene
LIN28B
Purity
>85%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
29.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 10% glycerol, 2mM DTT
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human LIN-28B Protein

  • CSDD2
  • CSDD2protein lin-28 homolog B
  • FLJ16517
  • lin-28 homolog B (C. elegans)
  • Lin-28.2
  • LIN28B
  • LIN-28B

Background

Lin28B belongs to the lin-28 family. LIN28 protein is a microRNA-binding protein that binds to and enhances the translation of the IGF-2 (insulin-like growth factor 2) mRNA. Lin28B is acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Recombinant human Lin28B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol LIN28B
Entrez