LIN-28B Antibody [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

LIN-28B Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human LIN-28B
  Val83-Thr250
  Accession # Q6ZN17
• Specificity: Detects human LIN-28B in direct ELISAs and Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human LIN-28A is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIN-28B Antibody [Alexa Fluor® 532]

• CSDD2
• CSDD2protein lin-28 homolog B
• FLJ16517
• lin-28 homolog B (C. elegans)
• Lin-28.2
• LIN28B
• LIN-28B

Background

Lin-28B (Lineage protein 28 homolog B) is a 35-37 kDa member of the Lin28 family of proteins. Although there is an accompanying Lin-28/28A molecule, Lin-28 and Lin-28B are not alternative splice forms but represent products of separate genes. Lin-28B is found in both the cytoplasm and nucleus of fetal hematopoietic stem and progenitor cells, embryonic liver and multiple tumor types. It is considered a regulator of miRNA activity. Active miRNAs arise from transcribed RNAs that are first cleaved by nuclear RNase III, next cleaved by cytoplasmic Dicer, and finally associate with Argonaute to generate a RISC RNA silencing complex. Lin-28A participates in the cytoplasmic uridylation of miRNAs on their 3’-end, blocking Dicer processing and initiating RNA degradation. Lin-28B, by contrast, acts in the nucleus and blocks the initial RNase III cleavage step. Human Lin-28B is 250 amino acids (aa) in length (SwissProt # Q6ZN17). It contains one CSD/cold shock domain (aa 29-102) plus two CCHC-type Zn-finger RNA bindingdomains (aa 127-144 and 149-166). Phosphorylation is known to occur on Ser105. There is one reported alternate start site at Met71, and there is a potential 11 aa substitution for aa 1-4. Two isoforms of LIN28B with apparent MWs of 22 kDa and 37 kDa have been reported. Over aa 83-250, human Lin-28B shares 84% aa sequence identity with mouse Lin-28B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.