LIN-28B Antibody (A2-4-5)

Western Blot: LIN-28B Antibody (A2-4-5) [NBP2-66933] - Analysis on Lin28B recombinant protein lysate using anti- Lin28B mouse mAb.

Immunocytochemistry/Immunofluorescence: LIN-28B Antibody (A2-4-5) [NBP2-66933] - Staining Lin28B in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. and counterstained with DAPI in order to highlight the nucleus (blue).

Product Discontinued

LIN-28B Antibody (A2-4-5) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human LIN-28B aa 73-250 / 250. (SwissProt: Q6ZN17 Human)
• Localization: Cytoplasm, nucleus
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LIN28B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for LIN-28B Antibody (A2-4-5)

• CSDD2
• CSDD2protein lin-28 homolog B
• FLJ16517
• lin-28 homolog B (C. elegans)
• Lin-28.2
• LIN28B
• LIN-28B

Background

Lin28B acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7),a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminaluridylation of pre-let-7. Urid

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LIN28B