LIMPII/SR-B2 Antibody - BSA Free

Western Blot: SR-BII Antibody [NB400-102] - Western blot analysis of SR-BII in 1. Human heart lysate 2. Mouse heart lysate

Immunocytochemistry/Immunofluorescence: SR-BII Antibody [NB400-102] - SR-BII antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).

• Reactivity: Hu, Mu, Rt, Bv, ChHa, Ha, Md
• Applications: WB, Flow, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

LIMPII/SR-B2 Antibody - BSA Free Summary

• Immunogen: A peptide containing residues from mouse SR-BII (between residues 400-478) plus an N-terminal cysteine was coupled to KLH. [UniProt# O35114]
• Marker: Lysosome Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SCARB2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry reported in scientific literature
  • Immunocytochemistry/ Immunofluorescence 1:25-1:100
  • Immunohistochemistry 1:25
  • Immunohistochemistry-Paraffin 1:25
  • Knockdown Validated reported in scientific literature (PMID 31132962)
  • Western Blot 1:1000
• Application Notes: In Western blot a band is observed at ~82 kDa in tissues that express SR-BII such as liver, testes, and adrenal glands. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  LIMPII/SR-B2 Peptide (NB400-102PEP)

Reactivity Notes

Mink.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for LIMPII/SR-B2 Antibody - BSA Free

• AMRF
• CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2(lysosomal integral membrane protein II)
• CD36 antigen
• CD36L2
• CD36L2LIMP II
• HLGP85
• LGP85
• LIMP-2,85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2
• LIMPII
• LIMPIICD36 antigen-like 2
• LPG85
• lysosome membrane protein 2
• Lysosome membrane protein II
• SCARB2
• Scavenger receptor class B member 2,85 kDa lysosomal membrane sialoglycoprotein
• scavenger receptor class B, member 2
• SRB2
• SR-B2
• SR-BII

Background

Scavenger receptor class BI or SCARB1 gene belongs to CD36 receptor family and encodes for at least two mRNA splice variants which are SR-BI and SR-BII, among which SR-BII variant is the main isoform in terms of mRNA levels in certain tissues. For example, SR-BII mRNA is 48-fold higher than SR-BI in brain cells and protein levels reaching 10-15% of SR-BI in the liver. Similar differential SR-BII protein expression levels have also been detected in considerable amounts in other tissues, such as testes, retinal pigment epithelial cells, and spleen. SR-BII isoform differs from SR-BI only in its entirely different, yet highly conserved, cytoplasmic C-terminus which has been proposed to contains a signal responsible for marked alterations in cellular distribution as well as cellular trafficking of SR-BII compared with SR-BI. In contrast to SR-BI, SR-BII mediates the rapid uptake of significant amounts of HDL into endosomal recycling compartment by a pathway which is different from selective lipid uptake at the cell surface (mediated by SR-BI). Because SR-BII influence cellular cholesterol trafficking/homeostasis in a manner distinct from SR-BI, the relative expression as well as functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIMPII/SR-B2 Antibody (NB400-102)(13)

Publications using NB400-102

• Zhang, X, Wang, H Et al. Enterovirus A71 Oncolysis of Malignant Gliomas. Mol Ther 2020-06-03 [PMID: 32304669] (IF/IHC, Mouse)
• Abad MA, Ruppert JG, Buzuk L et al. Endophilin-A2-mediated endocytic pathway is critical for enterovirus 71 entry into caco-2 cells Emerg Microbes Infect 2019-05-29 [PMID: 31132962] (KD, WB, Human)
• Pelletier RM, Akpovi CD, Chen L et al. Cholesterol metabolism and Cx43, Cx46, and Cx50 gap junction protein expression and localization in normal and diabetic and obese ob/ob and db/db mouse testes Am J Physiol Endocrinol Metab. 2017-12-31 [PMID: 28851737] (WB, Mouse)
• Marco S, Pujol A, Roca C et al. Progressive neurologic and somatic disease in a novel model of human Mucopolysaccharidosis type IIIC. Dis Model Mech. 2016-08-04 [PMID: 27491071] (IF/IHC, Mouse)
• Lobo M, Arenas M, Huerta L et al. Liver growth factor (LGF) induces testicular regeneration in EDS-treated rats and increases protein levels of class B scavenger receptors. Am. J. Physiol. Endocrinol. Metab. 2014-11-11 [PMID: 25389365] (WB, Rat)
• Casado ME, Huerta L, Ortiz AI et al. HSL-knockout mouse testis exhibits class B scavenger receptor upregulation and disrupted lipid raft microdomains J Lipid Res 2012-12-01 [PMID: 22988039]
• Truong TQ, Aubin D, Falstrault L et al. SR-BI, CD36, and caveolin-1 contribute positively to cholesterol efflux in hepatic cells. Cell Biochem Funct 2010-08-01 [PMID: 20629037]
• Barth H, Schnober EK, Neumann-Haefelin C et al. Scavenger receptor class B is required for hepatitis C virus uptake and cross-presentation by human dendritic cells. J Virol;82(7):3466-79. 2008-04-01 [PMID: 18216094] (FLOW, Human)
• Zhang, X et al. Regulation of alternative splicing of liver scavenger receptor class B gene by estrogen and the involved regulatory splicing factors. Endocrinology;148(11):5295-304. 2007-11-01 [PMID: 17673517] (WB, Rat)
• Huby, T, Doucet, C, Dachet, C, Ouzilleau, B, Ueda, Y, Afzal, V, Rubin, E, Chapman, MJ, Lesnik, P. Knockdown expression and hepatic deficiency reveal an atheroprotective role for SR-BI in liver and peripheral tissues. J Clin Invest;116(10):2767-76. 2006-10-01 [PMID: 16964311]
• Akpovi, CD et al. The predominance of one of the SR-BI isoforms is associated with increased esterified cholesterol levels not apoptosis in mink testis. J Lipid Res;47(10):2233-47. 2006-10-01 [PMID: 16861621] (WB, IHC-P, Mustelid)
• Kocher, O et al. Targeted disruption of the PDZK1 gene in mice causes tissue-specific depletion of the high density lipoprotein receptor scavenger receptor class B type I and altered lipoprotein metabolism. J Biol Chem;278(52):52820-5. 2003-12-26 [PMID: 14551195] (WB, Mouse)
• Wadsack, C et al. Scavenger receptor class B, type I on non-malignant and malignant human epithelial cells mediates cholesteryl ester-uptake from high density lipoproteins. Int J Biochem Cell Biol;35(4):441-54. 2003-04-01 [PMID: 12565706] (ICC/IF, Human, Chinese Hamster)

Bioinformatics

• Gene Symbol: SCARB2
• Entrez:
  • 950(Human)
  • 12492(Mouse)
  • 117106(Rat)
• Uniprot:
  • O35114()