LIM Kinase 1 [p Thr508] Antibody

Western Blot: LIM Kinase 1 [p Thr508] Antibody [NB100-82021] - Western blot analysis of lysates from COLO205 cells, using NB100-82021. The lane on the right is blocked with the phospho peptide.

Immunohistochemistry-Paraffin: LIM Kinase 1 [p Thr508] Antibody [NB100-82021] - Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using NB100-82021(left). The picture on the right is blocked with the phospho-peptide.

Western Blot: LIM Kinase 1 [p Thr508] Antibody [NB100-82021] - Western Blot analysis of HEPG2 using NB100-82021. Antibody was diluted at 1:1000

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

LIM Kinase 1 [p Thr508] Antibody Summary

• Description: Novus Biologicals Rabbit LIM Kinase 1 [p Thr508] Antibody (NB100-82021) is a polyclonal antibody validated for use in IHC and WB. Anti-LIM Kinase 1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human LIM Kinase 1 around the phosphorylation site of threonine 508 (R-Y-TP-V-V).
• Modification: p Thr508
• Specificity: Detects endogenous levels of LIM Kinase 1 only when phosphorylated at threonine 508.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LIMK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:300
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:2000
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  LIM Kinase 1 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25721396)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% glycerol, 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LIM Kinase 1 [p Thr508] Antibody

• EC 2.7.11.1
• LIM domain kinase 1
• LIM Kinase 1
• LIM motif-containing protein kinase
• LIMK
• LIMK1
• LIMK-1
• LIMKLIMK-1

Background

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase 1 and LIM kinase 2 belong to a small subfamily with a unique combination of 2 N terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Two splice variant have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIM Kinase 1 Antibody (NB100-82021)(1)

Publication using NB100-82021

• Gupta SC, Yadav R, Pavuluri R et al. Essential role of GluD1 in dendritic spine development and GluN2B to GluN2A NMDAR subunit switch in the cortex and hippocampus reveals ability of GluN2B inhibition in correcting hyperconnectivity Neuropharmacology. 2015-02-24 [PMID: 25721396] (WB, Mouse)

Bioinformatics

• Gene Symbol: LIMK1
• Entrez:
  • 3984(Human)
• Uniprot:
  • P53667(Human)