Human LIGHT/TNFSF14 ELISA Kit (Colorimetric) Summary
| Sensitivity |
15 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
TNFSF14 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
TNFSF14 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNFSF14. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Assay Diluent A (Item D): animal serum with 0.09% sodium azide aspreservative. For Standard/ Sample (serum/plasma) diluent.
- Assay Diluent B (Item E): 5x concentrated buffer. For Standard/Sample(cell culture medium/urine) diluent.
- Detection Antibody Light (Item F): biotinylated anti-human Light (each vialis enough to assay half microplate).
- HRP-Streptavidin Concentrate (Item G): 300x concentratedHRP-conjugated streptavidin.
- Light Microplate (Item A): coated with anti-human Light.
- Standards (Item C): recombinant human Light.
- Stop Solution (Item I): 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3,3’,5,5’-tetramethylbenzidine(TMB) in buffered solution.
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human LIGHT/TNFSF14 ELISA Kit (Colorimetric)
Background
TNFSF14 is encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for LIGHT/TNFSF14 ELISA Kit (KA1741). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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