LIGHT/TNFSF14 Antibody (906909) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) mouse LIGHT/TNFSF14 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB17943) followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB005). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 906909
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

LIGHT/TNFSF14 Antibody (906909) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse LIGHT/TNFSF14
  Asp72-Val239
  Accession # Q9QYH9
• Specificity: Detects mouse LIGHT/TNFSF14 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIGHT/TNFSF14 Antibody (906909) [Unconjugated]

• CD258 antigen
• CD258
• delta transmembrane LIGHT
• Herpes virus entry mediator ligand
• Herpesvirus entry mediator ligand
• HVEM-L
• HVEMLherpesvirus entry mediator-ligand
• ligand for herpesvirus entry mediator
• LIGHT
• LIGHTherpesvirus entry mediator A
• LTg
• TNFSF14
• TR2
• tumor necrosis factor (ligand) superfamily, member 14
• tumor necrosis factor ligand superfamily member 14
• tumor necrosis factor receptor-like 2
• tumor necrosis factor superfamily member LIGHT

Background

LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) is a member of the TNF superfamily and is designated TNFSF14. The gene for mouse LIGHT encodes a 239 amino acid residue (aa) type II transmembrane glycoprotein that contains a 37 aa N-terminal cytoplasmic domain, a 21 aa transmembrane region, and a 181 aa extracellular domain. A soluble form of mouse LIGHT is generated from the membrane form by proteolytic processing. Similar to other TNF ligand family members, LIGHT is assembled as a homotrimer. Mouse and human LIGHT share 71% aa sequence identity. LIGHT is expressed by activated lymphocytes, natural killer cells, immature dendritic cells, monocytes and granulocytes. Mouse LIGHT binds and signals via two distinct TNF receptor superfamily members, including the herpes virus entry mediator (HVEM/TNFRSF14) and the lymphotoxin beta receptor (LT beta R/TNFRSF3). In humans, LIGHT also binds the soluble human decoy receptor 3 (DcR3/TNFRSF6B). Signaling from LT beta R, which also binds LT alpha beta , induces apoptosis and the production of various cytokines. LIGHT-LT beta R signaling also plays a role in mesenteric lymph node organogenesis, and restoration of secondary lymphoid structure and function. Signaling from HVEM, which also binds LT alpha , co-stimulates T-helper cell type 1 (TH1) immune responses, enhances Cytotoxic T Lymphocytes (CTL)-mediated tumor immunity, and regulates allogeneic T cell activation and allograft rejection. Blockade of LIGHT-HVEM signaling has been shown to prevent graft versus host disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9QYH9()