LIGHT/TNFSF14 Antibody (4E3)

Western Blot: LIGHT/TNFSF14 Antibody (4E3) [H00008740-M01] - Analysis of TNFSF14 expression in transfected 293T cell line by TNFSF14 monoclonal antibody (M01), clone 4E3.Lane 1: TNFSF14 transfected lysate(22.4 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: LIGHT/TNFSF14 Antibody (4E3) [H00008740-M01] - Analysis of monoclonal antibody to TNFSF14 on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.

Immunoprecipitation: LIGHT/TNFSF14 Antibody (4E3) [H00008740-M01] - Analysis of TNFSF14 transfected lysate using anti-TNFSF14 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TNFSF14 MaxPab rabbit polyclonal antibody.

ELISA: LIGHT/TNFSF14 Antibody (4E3.) [H00008740-M01] - LIGHT/TNFSF14 Antibody (4E3) [H00008740-M01] - Detection limit for recombinant GST tagged TNFSF14 is approximately 1ng/ml as a capture antibody.

Product Discontinued

LIGHT/TNFSF14 Antibody (4E3) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TNFSF14 (AAH18058, 61 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LHWRLGEMVTRLPDGPAGSWEQLIQERRSHEVNPAAHLTGANSSLTGSGGPLLWETQLGVAFLRGLSYHDGALVVTKAGYYYIYSKVQLGGVGCPLGLASTITHGLYKRT
• Specificity: TNFSF14 - tumor necrosis factor (ligand) superfamily, member 14
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFSF14
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LIGHT/TNFSF14 Antibody (4E3)

• CD258 antigen
• CD258
• delta transmembrane LIGHT
• Herpes virus entry mediator ligand
• Herpesvirus entry mediator ligand
• HVEM-L
• HVEMLherpesvirus entry mediator-ligand
• ligand for herpesvirus entry mediator
• LIGHT
• LIGHTherpesvirus entry mediator A
• LTg
• TNFSF14
• TR2
• tumor necrosis factor (ligand) superfamily, member 14
• tumor necrosis factor ligand superfamily member 14
• tumor necrosis factor receptor-like 2
• tumor necrosis factor superfamily member LIGHT

Background

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIGHT/TNFSF14 Antibody (H00008740-M01)(1)

Publication using H00008740-M01

• Tsunahiko H, Kazuto M, Keiji O et al. Abundant TNF-LIGHT expression in the airways of patients with asthma with persistent airflow limitation: Association with nitrative and inflammatory profiles. Respir Investig. 2021-07-07 [PMID: 34244107]

Bioinformatics

• Gene Symbol: TNFSF14
• Entrez:
  • 8740(Human)
• OMIM:
  • 604520(Human)