Lewis A Blood Group Antigen Antibody (SPM522)


Immunohistochemistry-Paraffin: ABO, Blood Group A Antigen Antibody (SPM522) [NBP2-45281] - Human Colon Carcinoma stained with Lewis A Monoclonal Antibody (SPM522).

Product Details

Reactivity Hu, MuSpecies Glossary
Applications Flow, IHC-P, ICC/IF

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Lewis A Blood Group Antigen Antibody (SPM522) Summary

Mucins isolated from ovarian cyst fluid
Cell membrane, cytoplasmic and extracellular
Recognizes a carbohydrate determinant of Gal 1-3(Fuc 1-4) GlcNAc which is blood group antigen Lewis A. It is present primarily on epithelial cells such as colon and kidneys. In the tumors and dedifferentiated tissues, decrease of Lewis A antigen was observed. Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
IgG1 Kappa
Protein A or G purified
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  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.

Packaging, Storage & Formulations

Store at 4C.
10mM PBS and 0.05% BSA
0.05% Sodium Azide
Protein A or G purified


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Gene Symbol ABO