Leptin R Antibody (55305) [Alexa Fluor™ Plus 647]

• Reactivity: Mu
• Clone: 55305
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor Plus 647

Leptin R Antibody (55305) [Alexa Fluor™ Plus 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Leptin R
  Leu21-Leu840 (predicted)
  Accession # P48356
• Specificity: Detects mouse Leptin R in ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Leptin R Antibody (55305) [Alexa Fluor™ Plus 647]

• B219
• CD295 antigen
• CD295
• DB
• DKFZp686B1731
• HuB219
• LEPR
• LEP-R
• Leptin R
• leptin receptor
• LeptinR
• OB R
• OB receptor
• OB-R
• OBRCD295

Background

Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-R_L) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-R_s) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript lacking a transmembrane domain and potentially encoding a soluble form of the receptor has also been described. Circulating soluble OB-R, complexed to leptin, has been detected in mouse serum. Serum soluble OB-R levels have been shown to increase during pregnancy. OB-R_L transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-R_s transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney and primitive hematopoietic cell populations. OB-R is encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype. Mouse OB-R long form encodes a 1162 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 817 aa residue extracellular domain, a 21 aa residue transmembrane domain and a 302 aa residue cytoplasmic domain. The extracellular domain of OB-R contains two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant murine soluble OB-R binds leptin with high affinity and is a potent leptin antagonist.

1. Tartaglia, L.A. et al. (1995) Cell 83:1263.
2. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
3. Lee, J. I. and J.M. Friedman (1996) Nature 379:632.
4. Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.
5. Gavrilova, O. et al. (1997) J. Biol. Chem. 272:30546.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.