LEO1 Antibody - BSA Free

Western Blot: LEO1 Antibody [NB100-79777] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiLeo1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu, Rt, Pm, Xp, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

LEO1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit LEO1 Antibody - BSA Free (NB100-79777) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 350 and 400 of human senescence down regulated leo1-like using the numbering given in TrEMBL entry Q8WVC0 (GeneID 123169).
• Predicted Species: Rat (100%), Primate (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LEO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:5000
• Control:

  LEO1 Overexpression Lysate

Reactivity Notes

X. laevis and Orangutan (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LEO1 Antibody - BSA Free

• Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• RDLReplicative senescence down-regulated leo1-like protein
• RNA polymerase-associated protein LEO1

Background

LEO1 is a component of the PAF/RNA polymerase II transcription complex. It interacts with parafibromin, PAF1 and CTR9. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LEO1
• Entrez:
  • 123169(Human)
• Uniprot:
  • Q8WVC0(Human)