LDLR Antibody (472413) [Unconjugated]

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human LDLR Monoclonal Antibody (Catalog # MAB2148, filled histogram) or isotype control antibody (MAB002, open histogram), followed by PE-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B).

A172 cells were stained with Mouse Anti-Human LDLR Monoclonal Antibody (Catalog # MAB2148, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins.

U-118-MG cells were stained with Mouse Anti-Human LDLR Monoclonal Antibody (Catalog # MAB2148, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, IP, CyTOF-ready, ELISA(Cap)
• Clone: 472413
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

LDLR Antibody (472413) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human LDLR
  Ala22-Arg788
  Accession # P01130
• Specificity: Detects human LDLR in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) LDLR, recombinant human LRP-5, or rmLRP-6 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LDLR
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human LDL R Biotinylated Antibody (Catalog number BAF2148)
  Standard: Recombinant Human LDL R (Catalog number 2148-LD)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LDLR Antibody (472413) [Unconjugated]

• FH
• FHC
• LDL R
• LDL receptor
• LDLCQ2
• LDLR
• low density lipoprotein receptor
• low-density lipoprotein receptor class A domain-containing protein 3
• low-density lipoprotein receptor

Background

The Low Density Lipoprotein Receptor (LDLR) is the founding member of the LDLR family of scavenger receptors (1, 2). This family contains transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta -propellers. Although members of the family were originally thought to be endocytic receptors, it is now clear that some members interact with adjacent cell-surface molecules, expanding their range of activities (2). Human LDLR is synthesized as an 860 amino acid (aa) precursor that contains a 21 aa signal sequence, a 767 aa extracellular region, a 22 aa transmembrane segment and a 50 aa cytoplasmic tail (3). The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats that bind ligand. Cysteine residues in this region participate in intrachain disulfide bonds. This region is followed by three EGF-like repeats with a beta -propeller YWTD containing motif. The EGF-like repeats are responsible for ligand bonding and dissociation. Finally, there is a 50 aa membrane proximal Ser/Thr-rich region that serves as a carbohydrate attachment point (1, 3, 4). There is extensive O-linked and modest N-linked glycosylation. Thus the receptor’s predicted molecular weight of 93 kDa is increased to a native molecular weight of 120-160 kDa (3, 4). Within the 50 aa cytoplasmic tail, there is an NPXY motif that links the receptor to clathrin pits (1). The extracellular region of human LDLR is 51% aa identical to the extracellular region of human VLDLR, and 79% aa identical to the extracellular region of mouse LDLR. LDLR is constitutively expressed and binds ApoB of LDL and ApoE of VLDL (5). It is responsible for clearing 70% of plasma LDL in liver (5). Mutations in the LDLR gene cause the autosomal dominant disorder, familial hypercholesterolemia (6).

1. Strickland, D.K. et al. (2002) Trends Endocrinol. Metab. 13:66.
2. Nykjaer, A. and T.E. Willnow (2002) Trends Cell Biol. 12:273.
3. Yamamoto, T. et al. (1984) Cell 39:27.
4. Davis, C.G. et al. (1986) J. Biol. Chem. 261:2828.
5. Defesche, J.C. (2004) Semin. Vasc. Med. 4:5.
6. Varret, M. et al. (2008) Clin Genet. 73:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LDLR Antibody (MAB2148)(14)

Publications using MAB2148

• Alcorn, MDH;Sun, C;Gilliland, TC;Lukash, T;Crasto, CM;Raju, S;Diamond, MS;Weaver, SC;Klimstra, WB; Three positively charged binding sites on the eastern equine encephalitis virus E2 glycoprotein coordinate heparan sulfate- and protein receptor-dependent infection Nature communications 2025-08-05 [PMID: 40764487] (Flow Cytometry, Human)
• Li, W;Plante, JA;Lin, C;Basu, H;Plung, JS;Fan, X;Boeckers, JM;Oros, J;Buck, TK;Anekal, PV;Hanson, WA;Varnum, H;Wells, A;Mann, CJ;Tjang, LV;Yang, P;Reyna, RA;Mitchell, BM;Shinde, DP;Walker, JL;Choi, SY;Brusic, V;Llopis, PM;Weaver, SC;Umemori, H;Chiu, IM;Plante, KS;Abraham, J; Shifts in receptors during submergence of an encephalitic arbovirus Nature 2024-07-24 [PMID: 39048821] (Flow Cytometry, Human)
• Simon G. Pfisterer, Ivonne Brock, Kristiina Kanerva, Iryna Hlushchenko, Lassi Paavolainen, Pietari Ripatti et al. Multiparametric platform for profiling lipid trafficking in human leukocytes Cell Reports Methods 2/28/2022 [PMID: 35474963]
• Sonia Nava-Salazar, Arturo Flores-Pliego, Giovanni Pérez-Martínez, Sandra Parra-Hernández, America Vanoye-Carlo, Francisco Ibarguengoitia-Ochoa et al. Resistin Modulates Low-Density Lipoprotein Cholesterol Uptake in Human Placental Explants via PCSK9 Reproductive Sciences 11/1/2022 [PMID: 35467263]
• LE Clark, SA Clark, C Lin, J Liu, A Coscia, KG Nabel, P Yang, DV Neel, H Lee, V Brusic, I Stryapunin, KS Plante, AA Ahmed, F Catterucci, TL Young-Pear, IM Chiu, PM Llopis, SC Weaver, J Abraham VLDLR and ApoER2 are receptors for multiple alphaviruses Nature, 2021-12-20;0(0):. 2021-12-20 [PMID: 34929721] (Flow Cytometry, Human)
• Y Masuda, S Yamaguchi, C Suzuki, T Aburatani, Y Nagano, R Miyauchi, E Suzuki, N Yamamura, K Nagatomo, H Ishihara, K Okuno, F Nara, G Matschiner, R Hashimoto, T Takahashi, T Nishizawa Generation and Characterization of a Novel Small Biologic Alternative to Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Antibodies, DS-9001a, Albumin Binding Domain-Fused Anticalin Protein J. Pharmacol. Exp. Ther., 2018-02-20;365(2):368-378. 2018-02-20 [PMID: 29463608] (ELISA Capture, Human)
• Delia Susan-Resi A novel mechanism causing familial hypercholesterolemia: The proprotein convertase subtilisin/kexin type 9-resistant Arg410Ser LDL receptor mutation J. Biol. Chem, 2016-12-20;0(0):. 2016-12-20 [PMID: 27998977] (Flow Cytometry, Human)
• Elodie Weider Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation J Biol Chem, 2016-06-08;0(0):. 2016-06-08 [PMID: 27284008] (IHC, Human)
• Matthew R. Zanotelli, Hamisha Ardalani, Jue Zhang, Zhonggang Hou, Eric H. Nguyen, Scott Swanson et al. Stable engineered vascular networks from human induced pluripotent stem cell-derived endothelial cells cultured in synthetic hydrogels Acta Biomaterialia 4/15/2016 [PMID: 26945632]
• Boucrot E, Ferreira A, Almeida-Souza L, Debard S, Vallis Y, Howard G, Bertot L, Sauvonnet N, McMahon H Endophilin marks and controls a clathrin-independent endocytic pathway. Nature, 2014-12-17;517(7535):460-5. 2014-12-17 [PMID: 25517094] (Functional Assay)
• Amirache F, Levy C, Costa C, Mangeot P, Torbett B, Wang C, Negre D, Cosset F, Verhoeyen E Mystery solved: VSV-G-LVs do not allow efficient gene transfer into unstimulated T cells, B cells, and HSCs because they lack the LDL receptor. Blood, 2014-02-27;123(9):1422-4. 2014-02-27 [PMID: 24578496] (Flow Cytometry, Human)
• Karta , Maya R, Wickert , Lisa E, Curran , Colleen, Gavala , Monica L, Denlinger , Loren C, Gern , James E, Bertics , Paul J Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages. J Allergy Clin Immunol, 2014-02-14;133(4):1227-30. 2014-02-14 [PMID: 24529411] (Flow Cytometry, Human)
• DeVay , Rachel M, Shelton , David L, Liang , Hong Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2). J Biol Chem, 2013-02-19;288(15):10805-18. 2013-02-19 [PMID: 23430252] (ELISA Development, Human)
• Poirier S, Mayer G, Poupon V, McPherson PS, Desjardins R, Ly K, Asselin MC, Day R, Duclos FJ, Witmer M, Parker R, Prat A, Seidah NG Dissection of the endogenous cellular pathways of PCSK9-induced low density lipoprotein receptor degradation: evidence for an intracellular route. J. Biol. Chem., 2009-07-27;284(42):28856-64. 2009-07-27 [PMID: 19635789] (Flow Cytometry, Western Blot, Human)

Bioinformatics

• Gene Symbol: LDLR
• Uniprot:
  • P01130()