LDB1 Antibody - BSA Free

Western Blot: LDB1 Antibody [NBP1-77832] - This antibody was used at a 1:1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4C. The membrane was washed and reacted with a 1:20,000 dilution of DyLight800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

LDB1 Antibody - BSA Free Summary

• Description: This affinity purified antibody is directed against the mouse LDB1protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified LDB1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of mouse LDB1 protein. (Uniprot: P70662)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LDB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • ELISA 1:425000
  • Western Blot 1:500-1:3000
• Application Notes: This affinity purified antibody has been tested for use in ELISA, western blot and CHIP. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB1 by western blotting in the appropriate cell lysate or extract. This antibody has been used in a ChIP assay using murine erythroleukemia (MEL) cells. The test sequence was the upstream enhancer of the GATA-1 gene; a putative LDB1 binding region as suggested by Orkin et al. Anti-LDB1 was used successfully in ChIP assays to precipitate a roughly 4-fold enrichment at the GATA1-HS1 enhancer element in DMSO-induced murine erythroleukemia cells. We suggest using 20ug for 10E8 cells for ChIP.

Reactivity Notes

A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, frog, chicken and rat based on 100% homology for the immunogen sequence. Cross reactivity with LDB1 homologues from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for LDB1 Antibody - BSA Free

• carboxy terminal LIM domain protein 2
• Carboxyl-terminal LIM domain-binding protein 2
• CLIM-2
• CLIM2hLdb1
• LDB-1
• LIM domain binding 1
• LIM domain-binding factor-1
• LIM domain-binding protein 1
• NLILIM domain-binding factor CLIM2
• Nuclear LIM interactor

Background

LDB1 is also known as CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor. The LIM-domain binding protein binds to the LIM domain of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors, both of which have essential roles in development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LDB1 Antibody (NBP1-77832)(2)

Publications using NBP1-77832

• Hsu Y, Pintacuda G, Liu R et al. Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia iScience 2023-04-01 [PMID: 37207277] (WB, Human)
• Pintacuda G, Hsu Y, Tsafou K et al. Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders Cell Genomics 2023-01-01 [PMID: 36950384]

Bioinformatics

• Gene Symbol: LDB1
• Uniprot:
  • NC_000085.5(Human)
  • P70662(Mouse)