Latent TGF-beta bp2/LTBP-2 Antibody [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 700

Latent TGF-beta bp2/LTBP-2 Antibody [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Latent TGF‑β bp2/LTBP‑2
  Gln36-Glu1821
  Accession # Q14767
• Specificity: Detects human Latent TGF‑ beta bp2/LTBP‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) LTBP-2 is observed, and less than 1% cross-reactivity with recombinant human LTBP-1 and rm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Latent TGF-beta bp2/LTBP-2 Antibody [Alexa Fluor® 700]

• C14orf141
• GLC3D
• Latent TGFbeta bp2
• Latent TGF-beta bp2
• latent transforming growth factor beta binding protein 2
• latent-transforming growth factor beta-binding protein 2
• LTBP2
• LTBP-2
• LTBP3chromosome 14 open reading frame 141
• MSTP031

Background

LTBP-2 (Latent TGF-beta Binding Protein 2; also C14orf141) is a 220-250 kDa secreted glycoprotein member of the LTBP family of proteins. In SDS-PAGE and under reducing conditions, LTBP-2 runs at 220-250 kDa; under nonreducing conditions, LTBP-2 runs between 205-220 kDa. Although it is described as being a TGF-beta binding protein, it does not possess the ability to bind covalently to TGF-beta . Instead, it appears to bind to heparin sulfate moieties on syndecan-4 and perlecan, plus fibrillin-1 and fibulin-5, suggesting a role in both elastic fiber formation and the maintenance of basement membrane integrity. In addition, LTPB-2 is now known to form a complex with PC5/6A intracellularly, and upon secretion, dampen PC5 activation in the extracellular environment. Cells known to express LTBP-2 include lens and nonpigmented ciliary epithelium, fibroblasts, vascular smooth muscle and chondrocytes. Mature human LTBP-2 is 1786 amino acids (aa) in length (aa 36-1821). It possesses three heparin-binding regions (aa 94-115, 232-249, and 344-354), plus 20 EGF-like domains (aa 187-1818) that contain four interspersed TGF-beta type binding segments. Over aa 36-1821, human LTBP-2 shares 80% aa sequence identity with mouse LTBP-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.