LARP6 Antibody - Azide and BSA Free

Western Blot: LARP6 Antibody [H00055323-B01P] - Analysis of LARP6 expression in transfected 293T cell line by LARP6 polyclonal antibody. Lane 1: LARP6 transfected lysate(54.01 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: LARP6 Antibody [H00055323-B01P] - Analysis of purified antibody to LARP6 on HeLa cell. (antibody concentration 10 ug/ml)

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

LARP6 Antibody - Azide and BSA Free Summary

• Immunogen: LARP6 (NP_060827, 1 a.a. - 491 a.a.) full-length human protein. MAQSGGEARPGPKTAVQIRVAIQEAEDVDELEDEEEGAETRGAGDPARYLSPGWGSASEEEPSRGHSGTTASGGENEREDLEQEWKPPDEELIKKLVDQIEFYFSDENLEKDAFLLKHVRRNKLGYVSVKLLTSFKKVKHLTRDWRTTAHALKYSVVLELNEDHRKVRRTTPVPLFPNENLPSKMLLVYDLYLSPKLWALATPQKNGRVQEKVMEHLLKLFGTFGVISSVRILKPGRELPPDIRRISSRYSQVGTQECAIVEFEEVEAAIKAHEFMITESQGKENMKAVLIGMKPPKKKPAKDKNHDEEPTASIHLNKSLNKRVEELQYMGDESSANSSSDPESNPTSPMAGRRHAATNKLSPSGHQNLFLSPNASPCTSPWSSPLAQRKGVSRKSPLAEEGRLNCSTSPEIFRKCMDYSSDSSVTPSGSPWVRRRRQAEMGTQEKSPGTSPLLSRKMQTADGLPVGVLRLPRGPDNTRGFHGHERSRACV
• Specificity: LARP6 - La ribonucleoprotein domain family, member 6,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: LARP6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LARP6 Antibody - Azide and BSA Free

• Acheron
• Achn
• death-associated LA motif protein
• FLJ11196
• La ribonucleoprotein domain family member 6
• La ribonucleoprotein domain family, member 6
• la-related protein 6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LARP6 Antibody (H00055323-B01P)(13)

Publications using H00055323-B01P

• Kyung-Il K, Chang-Il K, Jeung-Hoon L et al. Inhibitory Effect of Mitoxantrone on Collagen Synthesis in Dermal Fibroblasts Ann Dermatol. 2022-05-20 [PMID: 35721328]
• Slocum E, Craig A, Villanueva A, Germain D et al. Parity predisposes breasts to the oncogenic action of PAPP-A and activation of the collagen receptor DDR2. Breast Cancer Res. 2019-05-02 [PMID: 31046834]
• Zhang Y, Stefanovic B. mTORC1 phosphorylates LARP6 to stimulate type I collagen expression. Sci Rep 2017-01-23 [PMID: 28112218]
• Zhang Y, Stefanovic B. Akt mediated phosphorylation of LARP6; critical step in biosynthesis of type I collagen. Sci Rep 2016-03-02 [PMID: 26932461] (WB)
• Stefanovic L, Longo L, Zhang Y et al. Characterization of binding of LARP6 to the 5' stem-loop of collagen mRNAs: Implications for synthesis of type I collagen RNA Biol. 2014-11-02 [PMID: 25692237] (WB, Human)
• Wang H, Stefanovic B. Role of LARP6 and Nonmuscle Myosin in Partitioning of Collagen mRNAs to the ER Membrane. PLoS One. 2014-10-01 [PMID: 25271881]
• Blackstock C, Higashi Y, Sukhanov S et al. Insulin-Like Growth Factor-1 Increases Synthesis of Collagen Type I via Induction of the mRNA-Binding Protein LARP6 Expression and Binding to the 5' Stem-loop of Col1a1 and Col1a2 mRNA. J Biol Chem. 2014-01-27 [PMID: 24469459]
• Vukmirovic M, Manojlovic Z, Stefanovic B. Serine-Threonine Kinase Receptor-Associated Protein (STRAP) Regulates Translation of Type I Collagen mRNAs. Mol Cell Biol. 2013-08-05 [PMID: 23918805]
• Manojlovic Z, Blackmon J, Stefanovic B. Tacrolimus (FK506) Prevents Early Stages of Ethanol Induced Hepatic Fibrosis by Targeting LARP6 Dependent Mechanism of Collagen Synthesis. PLoS One. 2013-06-03 [PMID: 23755290]
• Manojlovic Z, Stefanovic B. A novel role of RNA helicase A in regulation of translation of type I collagen mRNAs. RNA. 2011-12-21 [PMID: 22190748]
• Challa AA, Stefanovic B. A Novel Role of Vimentin Filaments: Binding and Stabilization of Collagen mRNAs. Mol Cell Biol. 2011-07-11 [PMID: 21746880]
• Cai L, Fritz D, Stefanovic L et al. Nonmuscle Myosin-Dependent Synthesis of Type I Collagen. J Mol Biol. 2010-07-13 [PMID: 20603131]
• Cai L, Fritz D, Stefanovic L, Stefanovic B. Binding of LARP6 to the Conserved 5' Stem-Loop Regulates Translation of mRNAs Encoding Type I Collagen. J Mol Biol. 2009-11-13 [PMID: 19917293]

Bioinformatics

• Gene Symbol: LARP6
• Entrez:
  • 55323(Human)
• Uniprot:
  • NP_060827(Human)