LAP3 Antibody - Azide and BSA Free

Western Blot: LAP3 Antibody [NBP2-97066] - Anti-LAP3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 56 kDa. Observed band size: 55 kDa

Immunocytochemistry/Immunofluorescence: LAP3 Antibody [NBP2-97066] - Immunofluorescence staining of LAP3 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human LAP3 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.

Immunohistochemistry-Paraffin: LAP3 Antibody [NBP2-97066] - Immunochemical staining of human LAP3 in human liver with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).

Immunohistochemistry-Paraffin: LAP3 Antibody [NBP2-97066] - Immunochemical staining of human LAP3 in human kidney with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).

Immunoprecipitation: LAP3 Antibody [NBP2-97066] - LAP3-His(C) was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate4 ul anti-LAP3-His(C) rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-LAP3-His(C) rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 56 kDa. Observed band size: 56 kDa

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

LAP3 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human LAP3 (Accession#: NP_056991.2; Met1-Ala519)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LAP3
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunocytochemistry/ Immunofluorescence 1:300-1:10000
  • Immunohistochemistry-Paraffin 1:10000-1:20000
  • Immunoprecipitation 1-4 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for LAP3 Antibody - Azide and BSA Free

• EC 3.4.11
• EC 3.4.11.1
• EC 3.4.11.5
• EC 3.4.13.23
• LAP
• LAP3
• LAPEP
• LAPEPEC 3.4.11.1
• leucine aminopeptidase 3Prolyl aminopeptidase
• Leucyl aminopeptidase
• PEPS
• PEPScytosol aminopeptidase
• Peptidase S
• Peptidase SLAP-3
• Proline aminopeptidase

Background

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAP3