LAP (TGF-beta 1) Antibody (860206) [Unconjugated]

Mouse splenocytes treated with 10 μg/mL Anti-CD3, 5 μg/mL Anti-CD28, 10 μg/mL Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and 20 μg/mL Recombinant Mouse IL‑2 (Catalog # 402-IL) for 24 hours to induce T regulatory cells (iTregs) were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse LAP (TGF‑ beta 1) Monoclonal Antibody (Catalog # MAB7666) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 860206
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

LAP (TGF-beta 1) Antibody (860206) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse LAP (TGF-beta 1)
  Met1-Ser390
  Accession # P04202
• Specificity: Detects mouse LAP (TGF-beta 1) in flow cytometry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TGFB1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LAP (TGF-beta 1) Antibody (860206) [Unconjugated]

• CED
• DPD1
• LAP (TGFbeta 1)
• LAP (TGF-beta 1)
• LAP
• TGFB
• TGFB1
• TGFbeta
• transforming growth factor beta 1

Background

TGF-beta 1 (Transforming Growth Factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF-beta isoform has some non‑redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa Latency‑Associated Peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99% aa identity with mouse, rat and horse TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta RI, also known as Activin Receptor‑Like Kinase 5(ALK‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors Betaglycan (also known as TGF‑ beta  RIII) and Endoglin, or use of Smad‑independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).

1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press, 29.
2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
5. Lin, J.S. et al. (2006) Reproduction 132:179.
6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
8. Derynck, R. et al. (1985) Nature 316:701.
9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LAP (TGF-beta 1) Antibody (MAB7666)(12)

Publications using MAB7666

• KJ Yang, WJ Choi, YK Chang, CW Park, SY Kim, YA Hong Inhibition of Xanthine Oxidase Protects against Diabetic Kidney Disease through the Amelioration of Oxidative Stress via VEGF/VEGFR Axis and NOX-FoxO3a-eNOS Signaling Pathway International Journal of Molecular Sciences, 2023-02-14;24(4):. 2023-02-14 [PMID: 36835220] (IHC, Mouse)
• K Hira, P Sharma, A Mahale, O Prakash Ku, A Sajeli Beg Cyclo(Val-Pro) and Cyclo(Leu-Hydroxy-Pro) from Pseudomonas sp. (ABS-36) alleviates acute and chronic renal injury under in vitro and in vivo models (Ischemic reperfusion and unilateral ureter obstruction) International immunopharmacology, 2021-12-29;103(0):108494. 2021-12-29 [PMID: 34973530] (Western Blot, Mouse)
• X Fan, ST Mills, MJ Kaalla, V Sueblinvon Alcohol induces TGF&beta1 via downregulation of miR-1946a in murine lung fibroblast Sci Rep, 2020-11-05;10(1):19089. 2020-11-05 [PMID: 33154445] (Neutralization, Mouse)
• HL Zheng, HY Zhang, CL Zhu, HY Li, S Cui, J Jin, SG Piao, YJ Jiang, MY Xuan, JZ Jin, YS Jin, JP Lee, BH Chung, BS Choi, CW Yang, C Li L-Carnitine protects against tacrolimus-induced renal injury by attenuating programmed cell death via PI3K/AKT/PTEN signaling Acta Pharmacol. Sin., 2020-06-17;0(0):. 2020-06-17 [PMID: 32555441] (IHC, Rat)
• S Dhital, NR Vyavahare Nanoparticle-based targeted delivery of pentagalloyl glucose reverses elastase-induced abdominal aortic aneurysm and restores aorta to the healthy state in mice PLoS ONE, 2020-03-27;15(3):e0227165. 2020-03-27 [PMID: 32218565] (IHC, Mouse)
• YH Khalifa, GM Mourad, WM Stephanos, SA Omar, RA Mehanna Bone Marrow-Derived Mesenchymal Stem Cell Potential Regression of Dysplasia Associating Experimental Liver Fibrosis in Albino Rats Biomed Res Int, 2019-11-06;2019(0):5376165. 2019-11-06 [PMID: 31781620] (Confocal Microscopy, Rat)
• CC Fang, CF Wu, YJ Liao, SF Huang, M Chen, YA Chen AAV serotype 8-mediated liver specific GNMT expression delays progression of hepatocellular carcinoma and prevents carbon tetrachloride-induced liver damage Sci Rep, 2018-09-14;8(1):13802. 2018-09-14 [PMID: 30217986] (IHC, Mouse)
• J Beumer, B Artegiani, Y Post, F Reimann, F Gribble, TN Nguyen, H Zeng, M Van den Bo, JH Van Es, H Clevers Enteroendocrine cells switch hormone expression along the crypt-to-villus BMP signalling gradient Nat. Cell Biol., 2018-07-23;20(8):909-916. 2018-07-23 [PMID: 30038251] (Bioassay, Mouse)
• S Downs-Cann, S Berkey, GM Delgoffe, RP Edwards, T Curiel, K Odunsi, DL Bartlett, N Obermajer Suppressive IL-17A(+)Foxp3(+) and ex-Th17 IL-17A(neg)Foxp3(+) Treg cells are a source of tumour-associated Treg cells Nat Commun, 2017-03-14;8(0):14649. 2017-03-14 [PMID: 28290453] (Neutralization, Mouse)
• HS Yu, MK Park, SA Kang, KS Cho, SJ Mun, HJ Roh Culture supernatant of adipose stem cells can ameliorate allergic airway inflammation via recruitment of CD4(+)CD25(+)Foxp3 T cells Stem Cell Res Ther, 2017-01-23;8(1):8. 2017-01-23 [PMID: 28114970] (Western Blot, Mouse)
• F Wirsdörfer, S de Leve, F Cappuccini, T Eldh, AV Meyer, E Gau, LF Thompson, NY Chen, H Karmouty-Q, U Fischer, M Kasper, D Klein, JW Ritchey, MR Blackburn, AM Westendorf, M Stuschke, V Jendrossek Extracellular Adenosine Production by ecto-5'-Nucleotidase (CD73) Enhances Radiation-Induced Lung Fibrosis Cancer Res., 2016-02-26;76(10):3045-56. 2016-02-26 [PMID: 26921334] (IHC, Mouse)
• Lee Y, Park H, Woo S, Park E, Kang J RhoA/phosphatidylinositol 3-kinase/protein kinase B/mitogen-activated protein kinase signaling after growth arrest-specific protein 6/mer receptor tyrosine kinase engagement promotes epithelial cell growth and wound repair via upregulation of hepatocyte growth factor in macrophages. J Pharmacol Exp Ther, 2014-06-17;350(3):563-77. 2014-06-17 [PMID: 24939420] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: TGFB1
• Uniprot:
  • P04202()