LAP (TGF-beta 1) Antibody (27240) [Biotin]

• Reactivity: Hu
• Applications: ELISA(Det)
• Clone: 27240
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: LYOPH

LAP (TGF-beta 1) Antibody (27240) [Biotin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)
  Leu30-Arg278 (Cys33Ser)
  Accession # P01137
• Specificity: Recognizes human LAP (TGF-beta 1) and human latent TGF-beta 1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TGFB1
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Human LAP TGF-beta 1 Antibody (Catalog # MAB2461)
  ELISA Detection: Human LAP TGF-beta 1 Biotinylated Antibody (Catalog # BAM2462)
  Standard: Recombinant Human LAP TGF-beta 1 (Catalog # 246-LP)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LAP (TGF-beta 1) Antibody (27240) [Biotin]

• CED
• DPD1
• LAP (TGFbeta 1)
• LAP (TGF-beta 1)
• LAP
• TGFB
• TGFB1
• TGFbeta
• transforming growth factor beta 1

Background

The TGF-beta family includes several related proteins (70 - 80% sequence homology) from mammalian, avain or Xenopus systems that are now designated TGF-beta 1, TGF-beta 2, TGF-beta 1.2, TGF-beta 3, TGF-beta 4 and TGF-beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta , that consists of TGF-beta associated non-covalently with LAP. These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF-beta family members are naturally associated with their own distinct LAPs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LAP (TGF-beta 1) Antibody (BAM2462)(5)

Publications using BAM2462

• Roy, A;Shi, L;Chang, A;Dong, X;Fernandez, A;Kraft, JC;Li, J;Le, VQ;Winegar, RV;Cherf, GM;Slocum, D;Poulson, PD;Casper, GE;Vallecillo-Zúniga, ML;Valdoz, JC;Miranda, MC;Bai, H;Kipnis, Y;Olshefsky, A;Priya, T;Carter, L;Ravichandran, R;Chow, CM;Johnson, MR;Cheng, S;Smith, M;Overed-Sayer, C;Finch, DK;Lowe, D;Bera, AK;Matute-Bello, G;Birkland, TP;DiMaio, F;Raghu, G;Cochran, JR;Stewart, LJ;Campbell, MG;Van Ry, PM;Springer, T;Baker, D; De novo design of highly selective miniprotein inhibitors of integrins ?v?6 and ?v?8 Nature communications 2023-09-13 [PMID: 37704610] (Bioassay, Human)
• Mitsuko Hara, Ikuyo Inoue, Yuta Yamazaki, Akiko Kirita, Tomokazu Matsuura, Scott L. Friedman et al. L(59) TGF-beta LAP degradation products serve as a promising blood biomarker for liver fibrogenesis in mice Fibrogenesis & Tissue Repair 2015-12-01 [PMID: 26379781]
• Cavassani KA, Campanelli AP, Moreira AP, Vancim JO, Vitali LH, Mamede RC, Martinez R, Silva JS Systemic and local characterization of regulatory T cells in a chronic fungal infection in humans. J. Immunol., 2006-11-01;177(9):5811-8. 2006-11-01 [PMID: 17056505] (Flow Cytometry, Human)
• Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Blood, 2004-09-16;105(2):750-8. 2004-09-16 [PMID: 15374887] (Flow Cytometry, Human)
• Kitani A, Fuss I, Pedersen A, Nawata H TGF-beta 1 plays an important role in the mechanism of CD4+CD25+ regulatory T cell activity in both humans and mice. J. Immunol., 2004-01-15;172(2):834-42. 2004-01-15 [PMID: 14707053] (Flow Cytometry, Human)

Review for LAP (TGF-beta 1) Antibody (BAM2462) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Flow Mouse 01/16/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 22939403
• Species: Mouse

Bioinformatics

• Gene Symbol: TGFB1
• Uniprot:
  • P01137(Human)