Langerin/CD207 Antibody

Langerin/CD207 was detected in immersion fixed paraffin-embedded sections of human skin using 15 µg/mL Goat Anti-Human Langerin/CD207 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2088) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of Langerhans cells in the skin epidermis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Langerin/CD207 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Langerin/CD207
  Tyr64-Pro328
  Accession # Q9UJ71
• Specificity: Detects human Langerin/CD207 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CD207
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Langerin/CD207 Antibody

• CD207 antigen
• CD207 antigen, langerin
• CD207 molecule, langerin
• CD207
• CLEC4KLangerhans cell specific c-type lectin
• C-type lectin domain family 4 member K
• C-type lectin domain family 4, member K
• Langerin

Background

Langerin (also known as CD207) is a type II transmembrane glycoprotein which is member K of the C-type lectin domain family 4 (1). Langerin is used as a marker for Langerhans cells (LCs) which represent the immature dendritic cells in the epidermis (1, 2). LCs uniquely contain “tennis racket”-shaped endosomal recycling compartment subdomains with pentalamellar membranes termed Birbeck granules (1-3). Langerin is necessary and sufficient for Birbeck granule formation (1). The 328 amino acid (aa) human langerin sequence contains a 43 aa cytoplasmic domain, a 21 aa transmembrane domain and a 264 aa extracellular domain (ECD) that contains a coiled-coil domain and a single C-type lectin domain. Trimerization greatly increases the lectin binding affinity (4). Langerin internalizes endogenous proteins such as type I procollagen. Internalization by LC is thought to lead to suppression of self reactions (4-6). Langerin also mediates endocytosis of non-peptide antigens containing mannose, N-acetyl glucosamine and fucose that are expressed by mycobacteria and fungae (4, 7). Some antigens, such as the M. leprae glycolipid arabinomycolate, are ultimately presented by human LC CD1a in cutaneous-draining lymph nodes (8). Langerin performs a barrier-like function to HIV-1 transmission due to its internalization of virus particles for destruction (9). A rare human polymorphism within the lectin domain, W264R, abolishes both carbohydrate recognition and Birbeck granule formation (10, 11). Genetic deletion of mouse langerin was not shown to have functional consequence other than abolishing Birbeck granule formation (12). Human langerin shares 68%, 62%, 71% aa identity with mouse, rat, and bovine langerin ECD, respectively.

1. Valladeau, J. et al. (2000) Immunity 12:71. 
2. Valladeau, J. et al. (2003) Immunol. Res. 28:93. 
3. McDermott, R. et al. (2002) Mol. Biol. Cell 13:317. 
4. Stambach, N.S. and M.E. Taylor (2003) Glycobiology 13:401. 
5. Tada, Y. et al. (2006) J. Invest. Dermatol. 126:1549. 
6. Ritter, U. and A. Osterloh (2007) Med. Microbiol. Immunol. 196:51. 
7. Takahara, K. et al. (2003) Int. Immunol. 16:819.
8. Hunger, R.E. et al. (2004) J. Clin. Invest. 113:701.
9. De Witte, L. et al. (2007) Nat. Med. 13:367.
10. Verdijk, P. et al. (2005) J. Invest. Dermatol. 124:714.
11. Ward, E.M. et al. (2006) J. Biol. Chem. 281:15450.
12. Kissenpfennig, A. et al. (2005) Mol. Cell. Biol. 25:88.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Langerin/CD207 Antibody (AF2088)(11)

Publications using AF2088

• E Cohen, J Mariotton, F Rozenberg, A Sams, TH van Kuppev, N Barry Delo, M Zerbib, M Bomsel, Y Ganor CGRP inhibits human Langerhans cells infection with HSV by differentially modulating specific HSV-1 and HSV-2 entry mechanisms Mucosal Immunology, 2022-05-13;0(0):. 2022-05-13 [PMID: 35562558] (Western Blot, Human)
• KM Bertram, NR Truong, JB Smith, M Kim, KJ Sandgren, KL Feng, JJ Herbert, H Rana, K Danastas, M Miranda-Sa, JW Rhodes, E Patrick, RC Cohen, J Lim, SL Merten, AN Harman, AL Cunningham Herpes Simplex Virus type 1 infects Langerhans cells and the novel epidermal dendritic cell, Epi-cDC2s, via different entry pathways PloS Pathogens, 2021-04-27;17(4):e1009536. 2021-04-27 [PMID: 33905459] (IHC, Human)
• SM Thornton, VD Samararatn, JG Skeate, C Buser, KP Lühen, JR Taylor, DM Da Silva, WM Kast The Essential Role of anxA2 in Langerhans Cell Birbeck Granules Formation Cells, 2020-04-15;9(4):. 2020-04-15 [PMID: 32326440] (Immunoelectron Microscopy, Human)
• KM Bertram, RA Botting, H Baharlou, JW Rhodes, H Rana, JD Graham, E Patrick, J Fletcher, TM Plasto, NR Truong, C Royle, CM Doyle, O Tong, N Nasr, L Barnouti, MP Kohout, AJ Brooks, MP Wines, P Haertsch, J Lim, MP Gosselink, G Ctercteko, JD Estes, MJ Churchill, PU Cameron, E Hunter, MA Haniffa, AL Cunningham, AN Harman Identification of HIV transmitting CD11c+ human epidermal dendritic cells Nat Commun, 2019-06-21;10(1):2759. 2019-06-21 [PMID: 31227717] (IHC, Human)
• AI Chiramel, NR Meyerson, KL McNally, RM Broeckel, VR Montoya, O Méndez-Sol, SJ Robertson, GL Sturdevant, KJ Lubick, V Nair, BH Youseff, RM Ireland, CM Bosio, K Kim, J Luban, VM Hirsch, RT Taylor, F Bouamr, SL Sawyer, SM Best TRIM5? Restricts Flavivirus Replication by Targeting the Viral Protease for Proteasomal Degradation Cell Rep, 2019-06-11;27(11):3269-3283.e6. 2019-06-11 [PMID: 31189110]
• Chemokine Levels in the Penile Coronal Sulcus Correlate with HIV-1 Acquisition and Are Reduced by Male Circumcision in Rakai, Uganda PLoS Pathog, 2016-11-29;12(11):e1006025. 2016-11-29 [PMID: 27898732] (IHC, Human)
• Taha Hirbod, Joshua Kimani, Annelie Tjernlund, Juliana Cheruiyot, Anna Petrova, Terry B. Ball et al. Stable CD4 Expression and Local Immune Activation in the Ectocervical Mucosa of HIV-Infected Women The Journal of Immunology 10/1/2013 [PMID: 24006463]
• Hirbod T, Kaldensjo T, Broliden K In situ distribution of HIV-binding CCR5 and C-type lectin receptors in the human endocervical mucosa. PLoS ONE, 2011-09-30;6(9):e25551. 2011-09-30 [PMID: 21984929] (IHC-Fr, Human)
• Bond E, Liang F, Sandgren KJ, Smed-Sorensen A, Bergman P, Brighenti S, Adams WC, Betemariam SA, Rangaka MX, Lange C, Wilkinson RJ, Andersson J, Lore K Plasmacytoid dendritic cells infiltrate the skin in positive tuberculin skin test indurations. J. Invest. Dermatol., 2011-08-18;132(1):114-23. 2011-08-18 [PMID: 21850028] (IHC-Fr, Human)
• Tove Kaldensjö, Pernilla Petersson, Anna Tolf, Gareth Morgan, Kristina Broliden, Taha Hirbod Detection of Intraepithelial and Stromal Langerin and CCR5 Positive Cells in the Human Endometrium: Potential Targets for HIV Infection PLoS ONE 6/29/2011 [PMID: 21738639]
• Peressin M, Holl V, Schmidt S, Decoville T, Mirisky D, Lederle A, Delaporte M, Xu K, Aubertin AM, Moog C HIV-1 replication in Langerhans and interstitial dendritic cells is inhibited by neutralizing and Fc-mediated inhibitory antibodies. J. Virol., 2010-11-17;85(2):1077-85. 2010-11-17 [PMID: 21084491] (Neutralization, Human)

Review for Langerin/CD207 Antibody (AF2088) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 01/06/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 20571487
• Species: Human

Bioinformatics

• Gene Symbol: CD207
• Uniprot:
  • Q9UJ71()