DEC-205/CD205 Antibody

Images

 
Western blot shows lysates of JAWSII mouse immature dendritic cell line and mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse DEC‑205/CD205 Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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DEC-205/CD205 Antibody Summary

Immunogen
E. coli-derived recombinant mouse DEC-205/CD205
Cys216-Pro503
Accession # Q60767
Specificity
Detects mouse DEC-205/CD205 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human DEC-205 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LY75
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF5975 in the following application:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DEC-205/CD205 Antibody

  • CD205
  • DEC205
  • DEC-205
  • Ly75

Background

DEC-205 (Dendritic and thymic Epithelial Cell-205; also Ly-75 and CD205) is a 200-205 kDa member of the mannose receptor family of molecules. In mouse, it is widely expressed, being found on B cells, Langerhans cells, bone marrow-derived dendritic cells, neutrophils, plus respiratory, intestinal, and thymic cortical epithelium. DEC-205 serves as a recognition/endocytic receptor for dying cells, and likely participates in the induction of self-tolerance. Mature mouse DEC-205 is a 1696 amino acid (aa) type I transmembrane glycoprotein (aa 28-1723). It contains a 1640 aa extracellular domain (ECD) (aa 28-1667) and a 31 aa cytoplasmic tail. The ECD is highly modular, containing a ricin B-type lectin domain (aa 33-182), a FN type II domain (aa 164-211) and nine sequential C-type lectin domains (aa 225-1662). There are at least two potential isoform variants. One shows a two aa substitution for aa 866-1723, and another shows a 64 aa substitution for aa 734-1723. Over aa 216-503, mouse DEC-205 shares 87% and 79% aa identity with rat and human DEC-205, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DEC-205/CD205 Antibody (AF5975) (0)

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Review for DEC-205/CD205 Antibody (AF5975) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF5975:
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Images Ratings Applications Species Date Details
Western Blot DEC-205/CD205 AF5975
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reviewed by:
Jesse Davidson
WB Mouse 07/27/2018
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Summary

ApplicationWestern Blot
Sample Testedprimary antibody
SpeciesMouse
LotCDOW011803A

Comments

CommentsAntibody used at 1:200
Blocking and incubation in TBST + 5% milk

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Recent Reviews

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Jesse Davidson
07/27/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol LY75
Uniprot