DEC-205/CD205 Antibody

Western blot shows lysates of JAWSII mouse immature dendritic cell line and mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse DEC‑205/CD205 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5975) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DEC‑205/CD205 at approximately 205 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

DEC-205/CD205 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse DEC-205/CD205
  Cys216-Pro503
  Accession # Q60767
• Specificity: Detects mouse DEC-205/CD205 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human DEC-205 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LY75
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DEC-205/CD205 Antibody

• CD205
• CD205;DCL-1 fusion;DEC-205;gp200;Ly-75;Lymphocyte antigen 75;membrane antigen MN;RP23-10L17.4
• DEC205
• DEC-205
• Ly75

Background

DEC-205 (Dendritic and thymic Epithelial Cell-205; also Ly-75 and CD205) is a 200-205 kDa member of the mannose receptor family of molecules. In mouse, it is widely expressed, being found on B cells, Langerhans cells, bone marrow-derived dendritic cells, neutrophils, plus respiratory, intestinal, and thymic cortical epithelium. DEC-205 serves as a recognition/endocytic receptor for dying cells, and likely participates in the induction of self-tolerance. Mature mouse DEC-205 is a 1696 amino acid (aa) type I transmembrane glycoprotein (aa 28-1723). It contains a 1640 aa extracellular domain (ECD) (aa 28-1667) and a 31 aa cytoplasmic tail. The ECD is highly modular, containing a ricin B-type lectin domain (aa 33-182), a FN type II domain (aa 164-211) and nine sequential C-type lectin domains (aa 225-1662). There are at least two potential isoform variants. One shows a two aa substitution for aa 866-1723, and another shows a 64 aa substitution for aa 734-1723. Over aa 216-503, mouse DEC-205 shares 87% and 79% aa identity with rat and human DEC-205, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for DEC-205/CD205 Antibody (AF5975) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Jesse Davidson WB Mouse 07/27/2018

Summary

• Application: Western Blot
• Sample Tested: primary antibody
• Species: Mouse
• Lot: CDOW011803A

Comments

• Comments: Antibody used at 1:200
  Blocking and incubation in TBST + 5% milk

Bioinformatics

• Gene Symbol: LY75
• Uniprot:
  • Q60767()