Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 488]

Product Image: Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 488] [DDX0362A488-100] - Vial of Alexa Fluor 488 conjugated antibody. Alexa Fluor 488 is optimally excited at 490 nm by the Blue laser (488 nm) and has an emission maximum of 525 nm.

• Reactivity: Hu, Mu, Rt, Po, Eq
• Applications: Flow, ICC/IF, IHC, NULL
• Clone: 929F3.01
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 488

Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 488] Summary

• Immunogen: Langerin/CD207 was used as immunogen.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CD207
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Product Image
• Application Notes: Permeabilization is recommended for flow cytometry.

Packaging, Storage & Formulations

• Storage: Store at -20C in the dark. Avoid freeze-thaw cycles.
• Buffer: PBS and 50% Glycerol
• Preservative: No Preservative
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 488]

• CD207 antigen
• CD207 antigen, langerin
• CD207 molecule, langerin
• CD207
• CLEC4KLangerhans cell specific c-type lectin
• C-type lectin domain family 4 member K
• C-type lectin domain family 4, member K
• Langerin

Background

Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Langerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Langerin/CD207 Antibody (DDX0362A488-100)(1)

Publication using DDX0362A488-100

• Baptista MA, Keszei M, Oliveira M et al. Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells. Nat Commun. 2016-07-18 [PMID: 27425374] (IF/IHC)
  
  Details:
  This citation used the Alexa Fluor 488 version of this antibody.

Bioinformatics

• Gene Symbol: CD207