Langerin/CD207 Antibody (923B7) - Azide and BSA Free

Immunohistochemistry-Frozen: Langerin/CD207 Antibody (923B7) [DDX0373P-100] - Langerin (red) stained human skin section at 20x magnification. Section counterstained with DAPI. Sections were fixed with 4% PFA PBS, permeabilized with 0.5% Triton TBS Ca/Azide, and blocked with 5% BSA TBS Ca/Azide prior to staining with anti-Langerin antibody diluted 1:100 at 37C for 1 hour. A secondary antibody was applied, and sections were counterstained with DAPI. Image from a verified customer review.

Immunohistochemistry: Langerin/CD207 Antibody (923B7) [DDX0373P-100] - IHC on human skin cryosectionswith 923B7-HRP

• Reactivity: Hu, Mu, Rt, Po
• Applications: Flow, IHC
• Clone: 923B7
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

Langerin/CD207 Antibody (923B7) - Azide and BSA Free Summary

• Immunogen: Langerin/CD207 was used as immunogen.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CD207
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Reactivity Notes

Human, Mouse, Rat, Porcine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl (pH 8.0)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for Langerin/CD207 Antibody (923B7) - Azide and BSA Free

• CD207 antigen
• CD207 antigen, langerin
• CD207 molecule, langerin
• CD207
• CLEC4KLangerhans cell specific c-type lectin
• C-type lectin domain family 4 member K
• C-type lectin domain family 4, member K
• Langerin

Background

Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Langerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Langerin/CD207 Antibody (DDX0373P-100)(10)

Publications using DDX0373P-100

• Nasr N et al. Inhibition of two temporal phases of HIV-1 transfer from primary Langerhans cells to T cells: the role of langerin. J Immunol 2014-09-01 [PMID: 25070850]
• Luckashenak N et al. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. J Immunol 2013-01-01 [PMID: 23209325]
• Hitzler M et al. Human Langerhans cells control Th cells via programmed death-ligand 1 in response to bacterial stimuli and nickel-induced contact allergy. PLoS One 2012-01-01 [PMID: 23056446]
• Iram N et al. Age-related changes in expression and function of Toll-like receptors in human skin. Development 2012-11-01 [PMID: 23034637]
• Segura E et al. Characterization of resident and migratory dendritic cells in human lymph nodes. J Exp Med 2012-04-09 [PMID: 22430490]
• Hervouet C et al. Langerhans cells prime IL-17-producing T cells and dampen genital cytotoxic responses following mucosal immunization. J Immunol 2010-05-01 [PMID: 20351191]
• Nfon CK et al. Langerhans cells in porcine skin. Vet Immunol Immunopathol 2008-12-15 [PMID: 18805593]
• Douillard P et al. Mouse lymphoid tissue contains distinct subsets of langerin/CD207 dendritic cells, only one of which represents epidermal-derived Langerhans cells. J Invest Dermatol 2005-11-01 [PMID: 16297200]
• Movassagh M et al. Selective accumulation of mature DC-Lamp+ dendritic cells in tumor sites is associated with efficient T-cell-mediated antitumor response and control of metastatic dissemination in melanoma. Cancer Res 2004-03-15 [PMID: 15026362]
• Valladeau J et al. Identification of mouse langerin/CD207 in Langerhans cells and some dendritic cells of lymphoid tissues. J Immunol 2002-01-15 [PMID: 11777972]

Review for Langerin/CD207 Antibody (DDX0373P-100) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Tyler Crowe IHC-Fr Human 01/18/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Frozen human skin sections
• Species: Human
• Lot: DDX0373-013

Comments

• Comments: Sections were fixed with 4% PFA PBS, permeabilized with 0.5% Triton TBS Ca/Azide, and blocked with 5% BSA TBS Ca/Azide prior to staining with anti-Langerin antibody diluted 1:100 at 37C for 1 hour. A secondary antibody was applied, and sections were counterstained with DAPI.

Bioinformatics

• Gene Symbol: CD207