Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free

Western Blot: Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free [NBP2-71114] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Laminin S.

Immunohistochemistry: Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free [NBP2-71114] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free [NBP2-71114] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI3B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free (NBP2-45583) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 314-561 of human LAMB2(NP_002283) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200
• Theoretical MW: 192.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free

• LAMB2
• Laminin B1s chain
• Laminin beta 2
• Laminin S
• laminin subunit beta-2
• laminin, beta 2 (laminin S)
• Laminin-11 subunit beta
• Laminin-14 subunit beta
• Laminin-15 subunit beta
• Laminin-3 subunit beta
• Laminin-4 subunit beta
• Laminin-7 subunit beta
• LAMSLaminin-9 subunit beta
• S-LAM beta
• S-laminin subunit beta

Background

The Laminins comprise a growing family of disulfide-linked heterotrimers consisting of three genetically distinct polypeptide chains, designated alpha, beta and gamma. A major component of the basal lamina, Laminins play a crucial role in providing a scaffolding upon which tissues are assembled and which serves as a physical barrier separating specialized tissues. During embryogenesis and early development, cells migrate along basement membranes, which are required for the polarization of cells. The beta-2 laminin chain plays a role in melanoma spread, promoting tumor migration along the abluminal surface of vessel, a phenomenon which has been termed extra-vascular migratory metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAMB2