Laminin S/Laminin beta 2 Antibody (CL2976) - Azide and BSA Free

Staining of human colon shows strong positivity in basement membrane of glandular epithelium.

Staining of human kidney shows strong positivity in renal glomeruli.

Staining of human heart shows strong membranous immunoreactivity in cardiomyocytes.

Staining of human cortex shows strong immunoreactivity in endothelial cells.

Staining of human lymph node shows absence of immunoreactivity in lymphoid cells (negative control).

Staining of human skeletal muscle shows strong membranous immunoreactivity in myocytes.

Analysis of purified human recombinant Laminin-421, Laminin-521, Laminin-332 and Laminin-111.

Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-Laminin S/Laminin beta 2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL2976
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Laminin S/Laminin beta 2 Antibody (CL2976) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Laminin S/Laminin beta 2 Antibody (CL2976) - Azide and BSA Free (NBP2-42386) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P55268, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMB2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Laminin S/Laminin beta 2 Antibody (CL2976) - Azide and BSA Free

• LAMB2
• Laminin B1s chain
• Laminin beta 2
• Laminin S
• laminin subunit beta-2
• laminin, beta 2 (laminin S)
• Laminin-11 subunit beta
• Laminin-14 subunit beta
• Laminin-15 subunit beta
• Laminin-3 subunit beta
• Laminin-4 subunit beta
• Laminin-7 subunit beta
• LAMSLaminin-9 subunit beta
• S-LAM beta
• S-laminin subunit beta

Background

The Laminins comprise a growing family of disulfide-linked heterotrimers consisting of three genetically distinct polypeptide chains, designated alpha, beta and gamma. A major component of the basal lamina, Laminins play a crucial role in providing a scaffolding upon which tissues are assembled and which serves as a physical barrier separating specialized tissues. During embryogenesis and early development, cells migrate along basement membranes, which are required for the polarization of cells. The beta-2 laminin chain plays a role in melanoma spread, promoting tumor migration along the abluminal surface of vessel, a phenomenon which has been termed extra-vascular migratory metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAMB2