Human Laminin gamma 1 ELISA Kit (Colorimetric)

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ELISA: Human Laminin gamma 1 ELISA Kit (Colorimetric) [NBP3-39758] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
Standard Curve Range
31.25 - 2000 pg/mL (example only; lot dependent)
Sensitivity
12.1 pg/mL (example only; lot dependent)

Order Details

Human Laminin gamma 1 ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
31.25 - 2000 pg/mL (example only; lot dependent)
Sensitivity
12.1 pg/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
LAMC1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human Laminin gamma 1 ELISA Kit (Colorimetric)

  • LAMB2Laminin-7 subunit gamma
  • LAMC1
  • Laminin B2 chain
  • Laminin B2
  • Laminin gamma 1
  • laminin subunit gamma-1
  • laminin, gamma 1 (formerly LAMB2)
  • Laminin-1 subunit gamma
  • Laminin-10 subunit gamma
  • Laminin-11 subunit gamma
  • Laminin-2 subunit gamma
  • Laminin-3 subunit gamma
  • Laminin-4 subunit gamma
  • Laminin-6 subunit gamma
  • Laminin-8 subunit gamma
  • Laminin-9 subunit gamma
  • MGC87297
  • S-LAM gamma
  • S-laminin subunit gamma

Background

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing Type IV Collagen networks, Nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol LAMC1