Laminin gamma 1 Antibody (CL3199) - Azide and BSA Free

Analysis in human placenta and tonsil tissues using NBP3-44435 antibody. Corresponding Laminin gamma 1 RNA-seq data are presented for the same tissues.

Staining of human kidney shows moderate positivity in basement membrane of cells in tubules and glomeruli.

Staining of human placenta shows strong positivity in basement membrane of trophoblastic cells.

Staining of human rectum shows moderate positivity in basement membrane of glandular cells.

Analysis in human lung tissue.

Analysis of purified human recombinant Laminin-111, Laminin-121, Laminin-211, Laminin-221, Laminin-411, Laminin-421, Laminin-511, Laminin-521 and Laminin-332.

Staining of rat rectum shows moderate positivity in basement membrane of glandular cells.

Staining of human tonsil shows no positivity in lymphoid cells as expected.

Staining of rat kidney shows moderate positivity in basement membrane of cells in tubules and glomeruli.

• Reactivity: Hu, Rt
• Applications: WB, IHC
• Clone: CL3199
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Laminin gamma 1 Antibody (CL3199) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of P11047, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMC1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Laminin gamma 1 Antibody (CL3199) - Azide and BSA Free

• LAMB2Laminin-7 subunit gamma
• LAMC1
• Laminin B2 chain
• Laminin B2
• Laminin gamma 1
• laminin subunit gamma-1
• laminin, gamma 1 (formerly LAMB2)
• Laminin-1 subunit gamma
• Laminin-10 subunit gamma
• Laminin-11 subunit gamma
• Laminin-2 subunit gamma
• Laminin-3 subunit gamma
• Laminin-4 subunit gamma
• Laminin-6 subunit gamma
• Laminin-8 subunit gamma
• Laminin-9 subunit gamma
• MGC87297
• S-LAM gamma
• S-laminin subunit gamma

Background

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing Type IV Collagen networks, Nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAMC1