Laminin alpha 4 Antibody (CL3183) - Azide and BSA Free

Analysis in human placenta and lymph node tissues using NBP3-44408 antibody. Corresponding Laminin alpha 4 RNA-seq data are presented for the same tissues.

Staining of human colon shows strong positivity in basement membrane of glandular cells.

Staining of human lymph node shows no positivity in non-germinal center cells as expected.

Staining of human placenta shows strong positivity in basement membrane of trophoblastic cells and endothelial cells.

Staining of human prostate shows moderate to strong membranous positivity in smooth muscle cells.

Staining of rat heart muscle shows moderate to strong membranous positivity in cardiomyocytes.

Staining of rat kidney shows strong membranous positivity in cells in glomeruli.

Staining of mouse stomach shows moderate positivity in basement membrane of glandular cells.

Staining of rat stomach shows moderate to strong positivity in basement membrane of glandular cells.

Staining of mouse epididymis shows moderate to strong membranous positivity in smooth muscle cells.

Analysis in human placenta tissue.

Analysis of purified human recombinant Laminin-411, Laminin-421, Laminin-511, Laminin-121, Laminin-221 and Laminin-332.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL3183
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Laminin alpha 4 Antibody (CL3183) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of Q16363, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMA4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Laminin alpha 4 Antibody (CL3183) - Azide and BSA Free

• DKFZp686D23145
• LAMA3LAMA4*-1
• LAMA4
• laminin alpha 4 chain
• Laminin alpha 4
• laminin subunit alpha-4
• laminin, alpha 4
• Laminin-14 subunit alpha
• Laminin-8 subunit alpha
• Laminin-9 subunit alpha

Background

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cellassociated extracellular matrices (ECMs)) are polymers of laminins with stabilizing type IV collagen networks, nidogen, and several proteoglycans.Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve, and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression, and tissue architecture. Each laminin is a heterotrimer of alpha, beta, and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and cognate laminin receptors. The human laminin alpha-4 gene maps to chromosome 6q21 and is expressed in adult heart, lung, ovary, small and large intestines, liver, and placenta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAMA4