Laminin alpha 4 Antibody (775830)

Laminin a4 was detected in perfusion fixed frozen sections of mouse stomach using Rat Anti-Mouse Laminin a4 Monoclonal Antibody (Catalog # MAB3837) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in gastric pits. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: IHC
• Clone: 775830
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Laminin alpha 4 Antibody (775830) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Laminin alpha 4
  Gln826-Ala1816 (predicted)
  Accession # P97927
• Specificity: Detects mouse Laminin alpha 4 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Laminin alpha 4 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: LAMA4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Laminin alpha 4 Antibody (775830)

• DKFZp686D23145
• LAMA3LAMA4*-1
• LAMA4
• laminin alpha 4 chain
• Laminin alpha 4
• laminin subunit alpha-4
• laminin, alpha 4
• Laminin-14 subunit alpha
• Laminin-8 subunit alpha
• Laminin-9 subunit alpha

Background

Laminins are heterotrimeric glycoproteins and are major components of the basement membrane. Each laminin is comprised of a single alpha , beta , and gamma chain that heterotrimerize via their coiled-coil domains to form a large cruciform-shaped molecule. Mature Laminin  alpha 4 is a subunit of Laminin 411, Laminin 421, and Laminin 423. Mature Laminin alpha 4 is a 1792 amino acid (aa) residue protein that has a Laminin N-terminal domain, four Laminin EGF-like domains, the coiled-coil domain, and five C‑terminal tandem Laminin G-like domains. The G-like domains contain binding sites for integrin, heparin, and dystroglycan. Within the region used as immunogen, mouse Laminin  alpha 4 shows 91% and 97% aa sequence identity with human and rat Laminin  alpha 4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Laminin alpha 4 Antibody (MAB3837)(2)

Publications using MAB3837

• V Lam, R Takechi, MJ Hackett, R Francis, M Bynevelt, LM Celliers, M Nesbit, S Mamsa, F Arfuso, S Das, F Koentgen, M Hagan, L Codd, K Richardson, B O'Mara, RK Scharli, L Morandeau, J Gauntlett, C Leatherday, J Boucek, JCL Mamo Synthesis of human amyloid restricted to liver results in an Alzheimer disease-like neurodegenerative phenotype PloS Biology, 2021-09-14;19(9):e3001358. 2021-09-14 [PMID: 34520451] (IHC, Mouse, Transgenic Mouse)
• L Li, MW Shirkey, T Zhang, Y Xiong, W Piao, V Saxena, C Paluskievi, YS Lee, N Toney, BM Cerel, Q Li, T Simon, KD Smith, KL Hippen, BR Blazar, R Abdi, JS Bromberg The lymph node stromal laminin alpha 5 shapes alloimmunity J. Clin. Invest., 2020-05-01;0(0):. 2020-05-01 [PMID: 32017712] (IHC, Mouse)

Bioinformatics

• Gene Symbol: LAMA4
• Uniprot:
  • P97927()