Laminin alpha 1 Antibody [Unconjugated]

U2OS human osteosarcoma cell line was stained with Goat Anti-Human Laminin alpha 1 Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF4187, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by APC-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Extracellular matrix (ECM) deposition in fetal bovine serum (FBS) or human platelet lysate (HPL)-cultured adipose-derived stem cell (ASC) sheets. (A) Gene expression of collagen 1 alpha 1 (COL1A1), fibronectin (FN1), and laminin (LAMA1) in ASCs under different culture condition (n = 3). (B) Sircol assay of ASCs cultured under different culture condition (n = 3). (C) Western blot analysis of the expression of the ECM proteins in FBS or HPL-cultured ASC sheets. (D) Venn diagram of the number of proteins detected on LC/MS in the ECM of ASC sheets cultured with FBS and HPL, respectively (*p < 0.05; **p < 0.01; ***p < 0.005 from control, #p < 0.05; ##p < 0.01; ###p < 0.005 between the indicated groups). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33195191), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Laminin alpha 1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Laminin alpha 1 N-Terminus Domain VI
  Leu22-Met269
  Accession # P25391
• Specificity: Detects human Laminin alpha 1 N-Terminus Domain VI in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse LAMA4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LAMA1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Laminin alpha 1 Antibody [Unconjugated]

• LAMA
• LAMA1 laminin, alpha 1
• LAMA1
• Laminin alpha 1
• S-LAM-alpha

Background

Laminin subunit alpha 1 (LAMA1) is a secreted 400 kDa extracellular matrix glycoprotein that contributes to the formation of basement membrane Laminin isoforms 1 and 3. It is one of three subunits ( alpha , beta , and gamma ) that interact via their coiled-coil domains to form the approximately 800 kDa cruciform, disulfide-linked, Laminin heterotrimer. The 3058 amino acid (aa) residue mature human alpha 1 chain contains an N-terminal Laminin VI domain (aa 18‑269), followed by domains V through III containing 17 EGF-like repeats, the coiled-coil domains II and I, and five globular, Laminin G-like domains. Over aa 22‑269, human Laminin alpha 1 shares 95% and 91% aa sequence identity with canine and mouse alpha 1 chain, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Laminin alpha 1 Antibody (AF4187)(4)

Publications using AF4187

• Judith R Reinhard, Emanuela Porrello, Shuo Lin, Pawel Pelczar, Stefano C Previtali, Markus A Rüegg Nerve pathology is prevented by linker proteins in mouse models for LAMA2-related muscular dystrophy PNAS Nexus 2023-04-03 [PMID: 37038437]
• Carl Granath, Hunter Noren, Hanna Björck, Nancy Simon, Kim Olesen, Sergey Rodin et al. Characterization of Laminins in Healthy Human Aortic Valves and a Modified Decellularized Rat Scaffold BioResearch Open Access 2020-12-07 [PMID: 33376633]
• Nai-Chen Cheng, Yuan-Kun Tu, Ning-Hsu Lee, Tai-Horng Young Influence of Human Platelet Lysate on Extracellular Matrix Deposition and Cellular Characteristics in Adipose-Derived Stem Cell Sheets Frontiers in Cell and Developmental Biology 2020-10-22 [PMID: 33195191] (Western Blot, Human)
• Judith R. Reinhard, Shuo Lin, Karen K. McKee, Sarina Meinen, Stephanie C. Crosson, Maurizio Sury et al. Linker proteins restore basement membrane and correct LAMA2 -related muscular dystrophy in mice Science Translational Medicine 2017-06-28 [PMID: 28659438]

Bioinformatics

• Gene Symbol: LAMA1
• Uniprot:
  • P25391()