Lamin A + C Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-19324] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Lamin A + C stained by Lamin A + C antibody diluted at 1:1000. Red: alpha Tubulin, stained by alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Mouse stomach. Lamin A + C stained by Lamin A + C antibody diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - C2C12 xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - RT2 xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Human breast carcinoma. Lamin A + C antibody diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Mahlarvu xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: Lamin A + C Antibody [NBP2-19324] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE NBP2-19324 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Lamin A + C antibody immunoprecipitates Lamin A + C protein in IP experiments.IP samples: HeLa whole cell extractA. 50 ug HeLa whole cell extractB. Control with 4 ug of preimmune Rabbit IgGC. Immunoprecipitation of Lamin A + C protein by 4 ug Lamin A + C antibody (NBP2-19324)7.5 % SDS-PAGEThe immunoprecipitated Lamin A + C protein was detected by Lamin A + C antibody (NBP2-19324) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]

Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (NBP2-19324) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (NBP2-19324) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Pm, RM
• Applications: WB, ICC/IF, IHC, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Lamin A + C Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit Lamin A + C Antibody - BSA Free (NBP2-19324) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-Lamin A + C Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Lamin A + C. The exact sequence is proprietary.
• Localization: Nucleus
• Marker: Nuclear Envelope Marker
• Predicted Species: Porcine (98%), Chimpanzee (100%), Bovine (98%), Rhesus Macaque (99%), Canine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LMNA
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Lamin A + C Antibody - BSA Free

• CDCD1
• CDDC
• CMD1A
• CMT2B1
• dilated 1A (autosomal dominant)
• EMD2
• FPLD
• HGPSFPL
• lamin A/C
• lamin A/C-like 1
• lamin-A/C
• LDP1
• LFP
• LGMD1B
• limb girdle muscular dystrophy 1B (autosomal dominant)
• LMN1IDC
• LMNC
• LMNL1
• prelamin-A/C
• PRO1,70 kDa lamin
• progeria 1 (Hutchinson-Gilford type)
• renal carcinoma antigen NY-REN-32

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Through alternate splicing, this gene encodes three type A lamin isoforms. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lamin A + C Antibody (NBP2-19324)(4)

Publications using NBP2-19324

• Yang JG, Sun B, Wang Z et al. Exosome-targeted delivery of METTL14 regulates NFATc1 m6A methylation levels to correct osteoclast-induced bone resorption Cell death & disease 2023-11-13 [PMID: 37957146]
• Brown SJ, Šolti? D, Synowsky SA et al. AAV9-mediated SMN gene therapy rescues cardiac desmin but not Lamin a/C and elastin dysregulation in Smn 2B/- spinal muscular atrophy mice Human molecular genetics 2023-07-27 [PMID: 37498175] (IHC-Fr, Mouse)
• Edara VV, Nooka S, Proulx J et al. beta-Catenin Regulates Wound Healing and IL-6 Expression in Activated Human Astrocytes Biomedicines 2020-11-06 [PMID: 33171974] (Human)
  
  Details:
  WES analysis of human beta-catenin knockdown astrocytes.
• Soltic D, Shorrock HK, Allardyce H et al. Lamin A/C dysregulation contributes to cardiac pathology in a mouse model of severe spinal muscular atrophy Hum. Mol. Genet. 2019-08-09 [PMID: 31397869]

Bioinformatics

• Gene Symbol: LMNA