Lamin A + C Antibody (4H7) - BSA Free

Immunoprecipitation: Lamin A + C Antibody (4H7) [NBP3-26167] - Immunoprecipitating Lamin A + C in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26167 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26167 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).

Immunohistochemistry: Lamin A + C Antibody (4H7) [NBP3-26167] - Image of Lamin A + C Antibody (4H7) diluted at 1:115 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry: Lamin A + C Antibody (4H7) [NBP3-26167] - Image of Lamin A + C Antibody (4H7) diluted at 1:115 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4H7) [NBP3-26167] - Staining of Hela cells with Lamin A + C Antibody (4H7) at 1:38, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: Lamin A + C Antibody (4H7) [NBP3-26167] - Overlay histogram showing Hela cells stained with Lamin A + C Antibody (4H7) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IHC, IP
• Clone: 4H7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Lamin A + C Antibody (4H7) - BSA Free Summary

• Description: Novus Biologicals Rabbit Lamin A + C Antibody (4H7) - BSA Free (NBP3-26167) is a recombinant monoclonal antibody validated for use in IHC, ELISA, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human Lamin A + C [UniProt P02545]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LMNA
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:200-1:1000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Lamin A + C Antibody (4H7) - BSA Free

• CDCD1
• CDDC
• CMD1A
• CMT2B1
• dilated 1A (autosomal dominant)
• EMD2
• FPLD
• HGPSFPL
• lamin A/C
• lamin A/C-like 1
• lamin-A/C
• LDP1
• LFP
• LGMD1B
• limb girdle muscular dystrophy 1B (autosomal dominant)
• LMN1IDC
• LMNC
• LMNL1
• prelamin-A/C
• PRO1,70 kDa lamin
• progeria 1 (Hutchinson-Gilford type)
• renal carcinoma antigen NY-REN-32

Background

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LMNA