Lamin A + C Antibody (4C4)

Images

 
Western Blot: Lamin A + C Antibody (4C4) [NBP2-25151] - Analysis of different cell lysates using mouse mAb to lamin A/C, NBP2-25151, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa, [3] HEK293 [4] C6, ...read more
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151] - Analysis of HeLa cells stained with mouse mAb to lamin A/C, NBP2-25151, dilution 1:2,000 in red, and costained with rabbit pAb to HSP60, ...read more
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151] - HeLa cells staing with NBP2-25151 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF
Clone
4C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Lamin A + C Antibody (4C4) Summary

Immunogen
Full length recombinant human lamin A expressed in and purified from E. coli. [UniProt# P02545]
Localization
Nucleus. Nucleus envelope.
Marker
Nuclear Envelope Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LMNA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunocytochemistry/Immunofluorescence 1:1000
Application Notes
This Lamin A + C (4C4) antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where bands can be seen at ~74 kDa (Lamin A) and ~65 kDa (Lamin C).
Theoretical MW
74/65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-25151 in the following applications:

  • WB
    1 publication

Reactivity Notes

Monkey and Mouse reactivity reported in scientific literature (PMID:31871052).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 50% glycerol (1:1)
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lamin A + C Antibody (4C4)

  • CDCD1
  • CDDC
  • CMD1A
  • CMT2B1
  • dilated 1A (autosomal dominant)
  • EMD2
  • FPLD
  • HGPSFPL
  • lamin A/C
  • lamin A/C-like 1
  • lamin-A/C
  • LDP1
  • LFP
  • LGMD1B
  • limb girdle muscular dystrophy 1B (autosomal dominant)
  • LMN1IDC
  • LMNC
  • LMNL1
  • prelamin-A/C
  • PRO1,70 kDa lamin
  • progeria 1 (Hutchinson-Gilford type)
  • renal carcinoma antigen NY-REN-32

Background

The Lamin proteins are members of the intermediate filament protein family but are located inside the nucleus rather than in the cytoplasm. The Lamins function as skeletal components tightly associated with the inner nuclear membrane. Originally the proteins of the nuclear cytoskeleton were named Lamin A, B and C, from top to bottom as visualized on SDS-PAGE gels. Subsequently it was found that Lamins A and C were coded for by a single gene, while the Lamin B band may contain two proteins encoded by two genes now called Lamin B1 and Lamin B2. Lamin A has a mass of about 74kDa while Lamin C is 65kDa. The Lamin A protein includes a C-terminal segment of 98 amino acids missing from Lamin C, while Lamin C has a unique C-terminal 6 amino acid peptide not present in Lamin A. Apart from these regions Lamin A and C are identical so that antibodies raised against either protein are likely to cross react with the other. Lamin polymerization and depolymerization is regulated by phosphorylation by cyclin dependent protein kinase 1 (CDK1), the key component of "maturation promoting factor", the central regulator of cell division. Activity of this kinase increases during cell division and is responsible for the breakdown of the nuclear lamina. Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome. This family of diseases belong to a larger group which are often referred to as Laminopathies, though some Laminopathies are associated in defects in Lamin B1, B2 or one or other of the numerous nuclear lamina binding proteins. A truncated version of Lamin A, commonly known as progerin, causes Hutchinson-Gilford progeria syndrome, a form of premature aging.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lamin A + C Antibody (NBP2-25151)(1)

We have publications tested in 2 confirmed species: Mouse, Monkey.

We have publications tested in 1 application: WB.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-25151AF405
Alexa Fluor 488 NBP2-25151AF488
Alexa Fluor 647 NBP2-25151AF647
Alexa Fluor 700 NBP2-25151AF700
Biotin NBP2-25151B
DyLight 350 NBP2-25151UV
DyLight 405 NBP2-25151V
DyLight 488 NBP2-25151G
DyLight 550 NBP2-25151R
DyLight 650 NBP2-25151C
DyLight 680 NBP2-25151FR
DyLight 755 NBP2-25151IR
FITC NBP2-25151F
HRP NBP2-25151H
Janelia Fluor 549 NBP2-25151JF549
Janelia Fluor 646 NBP2-25151JF646

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Bioinformatics

Gene Symbol LMNA
Entrez
OMIM
Uniprot