Lamin A + C Antibody (131C3) - BSA Free

Western Blot: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunoblotting result of NBP1-97687(131C3) recognizing nuclear lamins A and C in human fibroblasts.

Immunohistochemistry-Frozen: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry on frozen tissue section of human skin showing nuclear lamina staining in epithelial and connective tissue cells.

Immunohistochemistry: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry of on frozen sections of swine skin showing strong positive staining in nuclei of the epidermal cells and to a lesser extent in the connective tissue.

Immunohistochemistry-Frozen: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry of NBP1-97687(131C3) on frozen sections of human colon showing strong positive staining in nuclei of the epithelial cells and to a lesser extent in the connective tissue.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ha, Sh
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 131C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Lamin A + C Antibody (131C3) - BSA Free Summary

• Description: The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
• Immunogen: Derived by fusion of P3/X63.Ag8.653 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with purified Rat liver lamins.
• Marker: Nuclear Envelope Marker
• Specificity: This antibody reacts with an epitope located between residues 319-566 in lamin A and C.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LMNA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100-1:200
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:100-1:200
  • Western Blot 1:100-1:1000
• Application Notes: This antibody is suitable for immunocytochemistry, immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
  
  This antibody is CyTOF ready..

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Lamin A + C Antibody (131C3) - BSA Free

• CDCD1
• CDDC
• CMD1A
• CMT2B1
• dilated 1A (autosomal dominant)
• EMD2
• FPLD
• HGPSFPL
• lamin A/C
• lamin A/C-like 1
• lamin-A/C
• LDP1
• LFP
• LGMD1B
• limb girdle muscular dystrophy 1B (autosomal dominant)
• LMN1IDC
• LMNC
• LMNL1
• prelamin-A/C
• PRO1,70 kDa lamin
• progeria 1 (Hutchinson-Gilford type)
• renal carcinoma antigen NY-REN-32

Background

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LMNA