Lactoperoxidase Antibody

Product Discontinued

Lactoperoxidase Antibody Summary

• Description: This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
• Immunogen: Lactoperoxidase [Bovine Milk]. Uniprot ID P80025
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: LPO
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000 - 1:20000
  • Immunoprecipitation 1:100
  • Western Blot 1:500 - 1:5000
• Application Notes: This product has been assayed against 1.0 ug of Lactoperoxidase [Bovine Milk] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:1,000 - 1:5,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Cross reactivity with Lactoperoxidase from other sources has not been tested.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Ion exchange chromatography
• Reconstitution Instructions: Reconstitute with 100 ul deionized water.

Alternate Names for Lactoperoxidase Antibody

• EC 1.11.1
• lactoperoxidase
• MGC129990
• MGC129991
• Salivary peroxidase
• SAPX
• SPOEC 1.11.1.7

Background

Lactoperoxidase is an antibacterial agent in cow milk. The heme protein lactoperoxidase (LPO), also referred to as salivary peroxidase (SPO), is an oxidoreductase secreted into milk. LPO belongs to the XPO subfamily of the peroxidase family. It is expressed in mammary and salivary glands, and in the presence of H2O2, LPO acts as a catalyst for the oxidation of many phenols and aromatic amines. It is crucial for protecting the lactating mammary gland and intestinal tract of newborn infants against microorganisms. LPO binds one calcium ion per heterodimer and one heme B (iron-protoporphyrin IX) group covalently per heterodimer. The LPO gene, which spans 28 kb, is similar in gene organization and sequence to the peroxidase genes MPO and EPX, suggesting the possibility that these genes evolved from a common ancestral gene. The LPO and MPO genes are arranged in a tail-to-tail manner on chromosome 17q23.1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LPO