PSMD11 Antibody

Images

 
Western Blot: PSMD11 Antibody [NBP1-46191] - Treatment of human skin fibroblasts with 6BIO activated proteostatic modules. Representative immunoblot analyses of the autophagy-related Beclin1 and the 26S proteasome ...read more
Independent Antibodies: Western Blot: PSMD11 Antibody [NBP1-46191] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. ...read more

Product Details

Summary
Reactivity Hu, Mu, Dr, Rt, Bv, Xp, ZeSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

PSMD11 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 175 and 225 of human Proteasome (Prosome, Macropain) 26S Subunit, non-ATPase, 11 using the numbering given in entry NP_002806.2 (GeneID 5717).
Predicted Species
Rat (100%), Xenopus (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMD11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
Publications
Read Publications using
NBP1-46191 in the following applications:

  • WB
    7 publications

Reactivity Notes

X. tropicalis (100%). Use in Drosophila reported in scientific literature (PMID:31002009).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PSMD11 Antibody

  • MGC3844,26S proteasome regulatory subunit RPN6
  • p44.5,26S proteasome regulatory subunit 9
  • proteasome (prosome, macropain) 26S subunit, non-ATPase, 11,26S proteasome regulatory subunit p44.5
  • Rpn6,26S proteasome regulatory subunit S9
  • S9,26S proteasome non-ATPase regulatory subunit 11

Background

PSMD11 (Proteasome 26S non-ATPase subunit 11), also known as S9, Rpn6 or p44.5, has multi-subunit protease complexes consisting of 20S subunits composed of four seven-numbered rings with two outer rings containing alpha subunits and two central rings composed of beta subunits, and 19S caps of 6 ATPase and 11 non-ATPase subunits. PSMD11 is the main proteolytic enzyme that functions in ATP-dependent degradation of ubiquitinated proteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSMD11 Antibody (NBP1-46191)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Drosophila.

We have publications tested in 1 application: WB.


Filter By Application
WB
(7)
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Filter By Species
Human
(2)
Mouse
(3)
Drosophila
(3)
All Species
Showing Publications 1 - 10 of 10.
Publications using NBP1-46191 Applications Species
Manola MS, Gumeni S, Trougakos IP Differential Dose- and Tissue-Dependent Effects of foxo on Aging, Metabolic and Proteostatic Pathways Cells Dec 18 2021 [PMID: 34944088] (WB, Drosophila) WB Drosophila
Dina E, Sklirou A, Chatzigeorgiou S et al. An enriched polyphenolic extract obtained from the by-product of Rosa damascena hydrodistillation activates antioxidant and proteostatic modules Phytomedicine Sep 1 2021 [PMID: 34619431] (WB, Human, Drosophila) WB Human, Drosophila
Nath SR, Yu Z, Gipson TA et al. Androgen receptor polyglutamine expansion drives age-dependent quality control defects and muscle dysfunction J. Clin. Invest. May 29 2018 [PMID: 29809168] (WB, Mouse) WB Mouse
Sklirou AD, Gaboriaud-Kolar N, Papassideri I et al. 6-bromo-indirubin-3'-oxime (6BIO), a Glycogen synthase kinase-3B inhibitor, activates cytoprotective cellular modules and suppresses cellular senescence-mediated biomolecular damage in human fibroblasts Sci Rep 2017 Sep 15 [PMID: 28916781]
Tsakiri E, Gaboriaud-Kolar N, Iliaki K et al. The indirubin derivative 6-Bromoindirubin-3'-oxime (6BIO) activates proteostatic modules, reprograms cellular bioenergetics pathways and exerts anti-aging effects. Antioxid. Redox Signal. Mar 2 2017 [PMID: 28253732]
Welk V, Coux O, Kleene V et al. Inhibition of Proteasome Activity Induces Formation of Alternative Proteasome Complexes. J. Biol. Chem. 2016 Apr 18 [PMID: 27129254] (WB, Human) WB Human
Berschneider K Connecting the functions of the proteasome and mitochondria in the lung Thesis 2016 (WB, Mouse) WB Mouse
Yazgili AS, Meul T, Welk V et al. In-gel proteasome assay to determine the activity, amount, and composition of proteasome complexes from mammalian cells or tissues STAR protocols Jun 18 2021 [PMID: 34027484]
Meul T, Berschneider K, Schmitt S et al. Mitochondrial Regulation of the 26S Proteasome Cell Rep Aug 25 2020 [PMID: 32846138] (WB, Mouse) WB Mouse
Tsakiri E N, Gumeni S et al. Proteasome dysfunction induces excessive proteome instability and loss of mitostasis that can be mitigated by enhancing mitochondrial fusion or autophagy. Autophagy Apr 19 2019 [PMID: 31002009] (WB, Drosophila) WB Drosophila

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PSMD11