RPS7 Antibody (3G4)

Western Blot: RPS7 Antibody (3G4) [H00006201-M03] - Analysis of RPS7 expression in NIH/3T3 (Cat # L018V1).

Immunocytochemistry/Immunofluorescence: RPS7 Antibody (3G4) [H00006201-M03] - Analysis of monoclonal antibody to RPS7 on HeLa cell. Antibody concentration 20 ug/ml

Immunohistochemistry-Paraffin: RPS7 Antibody (3G4) [H00006201-M03] - Analysis of monoclonal antibody to RPS7 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml

Western Blot: RPS7 Antibody (3G4) [H00006201-M03] - Analysis of RPS7 expression in HeLa (Cat # L013V1).

Western Blot: RPS7 Antibody (3G4) [H00006201-M03] - Analysis of RPS7 expression in PC-12 (Cat # L012V1).

Western Blot: RPS7 Antibody (3G4) [H00006201-M03] - Analysis of RPS7 expression in Raw 264.7 (Cat # L024V1).

Sandwich ELISA: RPS7 Antibody (3G4) [H00006201-M03] - Detection limit for recombinant GST tagged RPS7 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
• Clone: 3G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

RPS7 Antibody (3G4) Summary

• Immunogen: RPS7 (NP_001002, 95 a.a. ~ 194 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IAQRRILPKPTRKSRTKNKQKRPRSRTLTAVHDAILEDLVFPSEIVGKRIRVKLDGSRLIKVHLDKAQQNNVEHKVETFSGVYKKLTGKDVNFEFPEFQL
• Specificity: RPS7 - ribosomal protein S7
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RPS7
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RPS7 Antibody (3G4)

• DBA8,40S ribosomal protein S7
• ribosomal protein S7

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S7E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RPS7 Antibody (H00006201-M03)(1)

Publication using H00006201-M03

• Nicholson J, Scherl A, Way L et al. A systems wide mass spectrometric based linear motif screen to identify dominant in-vivo interacting proteins for the ubiquitin ligase MDM2. Cell Signal 2014-02-28 [PMID: 24583282]

Bioinformatics

• Gene Symbol: RPS7
• Entrez:
  • 6201(Human)
• OMIM:
  • 603658(Human)
• Uniprot:
  • NP_001002(Human)