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Proteasome 19S S7 Antibody

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Western Blot: Proteasome 19S S7 Antibody [NBP1-87797] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Western Blot: Proteasome 19S S7 Antibody [NBP1-87797] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: Proteasome 19S S7 Antibody [NBP1-87797] - Staining of human hippocampus shows strong nuclear positivity in neuronal cells.
Simple Western: Proteasome 19S S7 Antibody [NBP1-87797] - Mouse whole spleen lysates at the indicated concentrations were probed with a 1:25 dilution of this Proteasome 19S S7 antibody. Burn-out of the major peak is ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Proteasome 19S S7 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RKTKEDEKDDKPIRALDEGDIALLKTYGQSTYSRQIKQVEDDIQQLLKKINELTGIKESDTGLAPPALWDLAA
Specificity
Specificity of human Proteasome 19S S7 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMC2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Simple Western
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Proteasome 19S S7 antibody validated for Simple Western from a verified customer review.
Control Peptide
Proteasome 19S S7 Protein (NBP1-87797PEP)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-87797 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Proteasome 19S S7 Antibody

  • 26S protease regulatory subunit 7
  • 26S proteasome AAA-ATPase subunit RPT1
  • MGC3004
  • MSS1ATPase, 2
  • proteasome (prosome, macropain) 26S subunit, ATPase, 2
  • Protein MSS1
  • putative protein product of Nbla10058

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: PSMC1 Antibody [NBP1-80959] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP1-80959] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
(1 Publication)
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Western Blot: RPS4X Antibody [NBP2-94118] - Analysis of extracts of various cell lines, using RPS4X at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: RPS4X Antibody [NBP2-94118] - Paraffin-embedded human kidney using RPS4X.
Species: Hu, Mu, Rt
Applications: WB, IHC
SUG1 Antibody
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Western Blot: SUG1 Antibody [NBP1-87372] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: SUG1 Antibody [NBP1-87372] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: SUG1 Antibody [NBP1-87372] - Staining of human testis.
Species: Hu, Mu, Rt
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RPS12 Antibody
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Western Blot: RPS12 Antibody [NBP2-94504] - Analysis of extracts of various cell lines, using RPS12 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Immunocytochemistry/Immunofluorescence: RPS12 Antibody [NBP2-94504] - Analysis of MCF-7 cells using RPS12. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: RPS12 Antibody [NBP2-94504] - Paraffin-embedded rat brain using RPS12.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
RPS3 Antibody
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Western Blot: RPS3 Antibody [NBP1-33691] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS3 antibody diluted at a dilution of 1:500.
Immunocytochemistry/Immunofluorescence: RPS3 Antibody [NBP1-33691] - Analysis of methanol-fixed HeLa, using RPS3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: RPS3 Antibody [NBP1-33691] - Mouse intestine diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
Proteasome 19S S3
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NBP2-73636
Western Blot: Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free [NBP2-73636] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S S3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Prot
Flow Cytometry: Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free [NBP2-73636] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Proteasome 19S S3 antibody, and then analyzed by flow cytometry.
Western Blot: Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free [NBP2-73636] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Proteasome 19S S3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Recombinant Human
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RPS9 Antibody
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Western Blot: RPS9 Antibody [NBP2-88192] - Host: Rabbit. Target Name: RPS9. Sample Type: Human MCF7. Antibody Dilution: 1.0ug/mlRPS9 is supported by BioGPS gene expression data to be expressed in MCF7
Immunohistochemistry-Paraffin: RPS9 Antibody [NBP2-88192] - Rabbit Anti-RPS9 antibody. Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver. Observed Staining: Cytoplasm in hepatocytes. Primary Antibody Concentration: 1:600. Secondary Antibody: Donkey anti-Rabbit-Cy3.
Western Blot: RPS9 Antibody [NBP2-88192] - WB Suggested Anti-RPS9 Antibody. Titration: 1.0 ug/ml. Positive Control: Hela Whole CellRPS9 is supported by BioGPS gene expression data to be expressed in HeLa
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Tbp7 Antibody
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Western Blot: Tbp7 Antibody [NBP1-87799] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
Immunocytochemistry/Immunofluorescence: Tbp7 Antibody [NBP1-87799] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Tbp7 Antibody [NBP1-87799] - Staining of human gall bladder shows nuclear positivity in glandular cells.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
PSMD11 Antibody
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Western Blot: PSMD11 Antibody [NBP1-46191] - Treatment of human skin fibroblasts with 6BIO activated proteostatic modules. Representative immunoblot analyses of the autophagy-related Beclin1 and the 26S proteasome subunits Rpn6 (PSMD11), Rpt6, alpha7 and beta5 expression levels after cell exposure to the shown concentrations of 6BIO for 36 h. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-017-11662-7), licensed under a CC-BY licence.
Western Blot: PSMD11 Antibody [NBP1-46191] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PSMD11 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PSMD11 was also immunoprecipitated by rabbit anti-PSMD11 antibody NBP1-46192, which recognizes a downstream epitope.
(6 Publications)
Species: Hu, Mu
Applications: WB, IP
Ribosomal Protein
S6/RPS6 Antibody
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Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS6 antibody NB100-1595 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.Previous
Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RPS6 (NB100-1595). Detection: DAB
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(1 Review)
(3 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
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Immunocytochemistry/Immunofluorescence: PSMD7 Antibody [NBP2-38780] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: PSMD7 Antibody [NBP2-38780] - Staining of human kidney.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
5-HT1B Antibody
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(1 Publication)
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RPS10 Antibody
NBP1-31357
Western Blot: RPS10 Antibody [NBP1-31357] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: RPS10 Antibody [NBP1-31357] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RPS10 protein stained by RPS10 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: RPS10 Antibody [NBP1-31357] - Paraffin-embedded mouse colon diluted at 1:500.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
PSMD6 Antibody
NBP1-81545
Western Blot: PSMD6 Antibody [NBP1-81545] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: PSMD6 Antibody [NBP1-81545] - Staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PSMD6 Antibody [NBP1-81545] - Staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
L1CAM Antibody
(UJ127.11)
NB100-2682
Western Blot: L1CAM Antibody (UJ127.11) [NB100-2682] - SUM159 cells were exposed to 20% or 1% O2 for 48 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. L1CAM Antibody (NB100-2682) primary antibody: 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: L1CAM Antibody (UJ127.11) [NB100-2682] - The left panel (A) shows untreated Neuro2a cells and the right panel (B) shows Neuro2a cells that were serum starved then treated with 1mM cAMP overnight to induce axon growth. Cells were fixed in 4% paraformaldehyde for 10 minutes and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti- NB100-2682 at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: L1CAM Antibody (UJ127.11) [NB100-2682] - Analysis of a FFPE tissue section of human brain using 1:200 dilution of L1CAM [UJ127.11] antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Cytoplasmic and membrane staining as observed in the cerebral cortex
(1 Review)
(1 Publication)
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, Flow-CS, IF
RPS5 Antibody (4H3)
H00006193-M02
Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - Analysis of RPS5 expression in transfected 293T cell line by RPS5 monoclonal antibody (M02), clone 4H3.Lane 1: RPS5 transfected lysate(22.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: RPS5 Antibody (4H3) [H00006193-M02] - Analysis of monoclonal antibody to RPS5 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - RPS5 monoclonal antibody (M02), clone 4H3 Analysis of RPS5 expression in HeLa.
(2 Publications)
Species: Hu, Mu, Rt, Pr
Applications: WB, ELISA, ICC/IF, S-ELISA
Rpn2 Antibody
NBP2-38323
Western Blot: Rpn2 Antibody [NBP2-38323] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/Immunofluorescence: Rpn2 Antibody [NBP2-38323] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & actin filaments.
Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human liver.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
PSMD8 Antibody
NBP1-83254
Western Blot: PSMD8 Antibody [NBP1-83254] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: PSMD8 Antibody [NBP1-83254] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: PSMD8 Antibody [NBP1-83254] - Staining of human testis shows high expression.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Proteasome 19S S7 Antibody (NBP1-87797) (0)

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Reviews for Proteasome 19S S7 Antibody (NBP1-87797) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-87797:
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WB
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Images Ratings Applications Species Date Details
Simple Western Proteasome 19S S7 NBP1-87797
Enlarge 		4
reviewed by:
Anonymous
 Simple Western Mouse 08/23/2019
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Summary

ApplicationSimple Western
Sample TestedMouse Spleen Lysate
SpeciesMouse
LotA107028
  5
reviewed by:
Anonymous
 WB Human 08/20/2015
View

Summary

ApplicationWestern Blot
Sample TestedHEK 293 cell lysate20 ug
SpeciesHuman

Blocking

Blocking Details5 % Non-fat milk for 20 minutes at RT

Primary Anitbody

Dilution Ratio1:800

Secondary Antibody

Secondary DescriptionGOAT ANTI-rabbit igG, HRP
Secondary Concentration1:10000

Details

Detection Noteschemiluminescent, GE 4000, 30sec

Product General Protocols

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FAQs for Proteasome 19S S7 Antibody (NBP1-87797) (0)

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Secondary Antibodies

 

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Simple Western Proteasome 19S S7 NBP1-87797
Enlarge
 Anonymous
08/23/2019
Application: Simple Western
Species: Mouse
read this review
 
 Anonymous
08/20/2015
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol PSMC2