![Western Blot: Proteasome 19S S7 Antibody [NBP1-87797] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize2/Proteasome-19S-S7-Antibody-Western-Blot-NBP1-87797-img0008.jpg "Western Blot: Proteasome 19S S7 Antibody [NBP1-87797] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: RKTKEDEKDDKPIRALDEGDIALLKTYGQSTYSRQIKQVEDDIQQLLKKINELTGIKESDTGLAPPALWDLAA |
| Specificity | Specificity of human, mouse, rat Proteasome 19S S7 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PSMC2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. |
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| Control Peptide |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||||||||
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reviewed by:
Anonymous |
WB | Human | 08/20/2015 |
Summary
Blocking
Primary Anitbody
Secondary Antibody
Details
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Secondary Antibodies |
Isotype Controls |
Diseases for Proteasome 19S S7 Antibody (NBP1-87797)Discover more about diseases related to Proteasome 19S S7 Antibody (NBP1-87797).
| Pathways for Proteasome 19S S7 Antibody (NBP1-87797)View related products by pathway.
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| Gene Symbol | PSMC2 |
![Immunohistochemistry-Paraffin: Proteasome 19S S7 Antibody [NBP1-87797] - Staining of human hippocampus shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize2/Proteasome 19S S7 Antibody-Immunohistochemistry-Paraffin-NBP1-87797-img0009.jpg "Immunohistochemistry-Paraffin: Proteasome 19S S7 Antibody [NBP1-87797] - Staining of human hippocampus shows strong nuclear positivity in neuronal cells.")
![Western Blot: Proteasome 19S S7 Antibody [NBP1-87797] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize2/Proteasome-19S-S7-Antibody-Western-Blot-NBP1-87797-img0006.jpg "Western Blot: Proteasome 19S S7 Antibody [NBP1-87797] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: Proteasome 19S S7 Antibody [NBP1-87797] - Staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize2/Proteasome-19S-S7-Antibody-Immunohistochemistry-Paraffin-NBP1-87797-img0001.jpg "Immunohistochemistry-Paraffin: Proteasome 19S S7 Antibody [NBP1-87797] - Staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.")
![Western Blot: PSMC1 Antibody [NBP1-80959] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize2/PSMC1 Antibody-Western Blot-NBP1-80959-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: PSMC1 Antibody [NBP1-80959] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize2/PSMC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80959-img0008.jpg)
![Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP1-80959] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize2/PSMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-80959-img0012.jpg)
![SDS-Page: Recombinant Human RPS4Y1 Protein [H00006192-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00006192-P01-1.jpg)
![Immunohistochemistry-Paraffin: RPS4X Antibody [NBP1-82761] - RPS4Y1 Antibody [NBP1-82761] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/RPS4X-Antibody-Immunohistochemistry-Paraffin-NBP1-82761-img0004.jpg)
![Western Blot: SUG1 Antibody (25D5) [NB100-345] - SUG1 antibody with 30 ug whole cell lysate run on SDS-PAGE. Lane A: 293T; B: A431; C: H1299; D: Hela; E: Hep G2; F: Molt-4; G: Raji 10% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize2/SUG1-Antibody-(25D5)-Western-Blot-NB100-345-img0002.jpg)
![Western Blot: RPS12 Antibody [NBP2-58885] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize2/RPS12 Antibody-Western Blot-NBP2-58885-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RPS12 Antibody [NBP2-58885] - Staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize2/RPS12-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58885-img0001.jpg)
![Western Blot: RPS3 Antibody [NBP1-33691] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS3 antibody diluted at a dilution of 1:500.](http://images.novusbio.com/fullsize2/RPS3-Antibody-Western-Blot-NBP1-33691-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: RPS3 Antibody [NBP1-33691] - Analysis of methanol-fixed HeLa, using RPS3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize2/RPS3 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33691-img0003.jpg)
![Immunohistochemistry-Paraffin: RPS3 Antibody [NBP1-33691] - Mouse intestine diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize2/RPS3-Antibody-Immunohistochemistry-Paraffin-NBP1-33691-img0009.jpg)
![Western Blot: Proteasome 19S S3 Antibody (2A2) [NBP2-01808] Analysis of extracts (35ug) from 9 different cell lines by using anti-Proteasome 19S S3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Proteasome 19S S3 Antibody (2A2)-Western Blot-NBP2-01808-img0005.jpg)
![Flow Cytometry: Proteasome 19S S3 Antibody (2A2) [NBP2-01808] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Proteasome 19S S3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Proteasome 19S S3 Antibody (2A2)-Flow Cytometry-NBP2-01808-img0003.jpg)
![Western Blot: Proteasome 19S S3 Antibody (2A2) [NBP2-01808] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S S3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 19S S3.](http://images.novusbio.com/fullsize/Proteasome 19S S3 Antibody (2A2)-Western Blot-NBP2-01808-img0004.jpg)
![SDS-Page: Recombinant Human RPS8 Protein [H00006202-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00006202-Q01-1.jpg)
![Western Blot: RPS9 Antibody [NBP2-22298] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22298 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize2/RPS9-Antibody-Western-Blot-NBP2-22298-img0001.jpg)
![Immunohistochemistry: RPS9 Antibody [NBP2-22298] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RPS9 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize2/RPS9-Antibody-Immunohistochemistry-NBP2-22298-img0006.jpg)
![Western Blot: Tbp7 Antibody [NBP1-87799] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize2/Tbp7 Antibody-Western Blot-NBP1-87799-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Tbp7 Antibody [NBP1-87799] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/Tbp7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87799-img0009.jpg)
![Immunohistochemistry-Paraffin: Tbp7 Antibody [NBP1-87799] - Staining of human gall bladder shows nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize2/Tbp7-Antibody-Immunohistochemistry-Paraffin-NBP1-87799-img0001.jpg)
![Western Blot: PSMD11 Antibody [NBP1-46191] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PSMD11 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PSMD11 was also immunoprecipitated by rabbit anti-PSMD11 antibody NBP1-46192, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize2/PSMD11-Antibody-Western-Blot-NBP1-46191-img0002.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of Human RPS6 on HeLa whole cell lysate using NB100-1595. RPS6 was also immunoprecipitated using rabbit anti-RPS6 antibodies NB100-1594 and another RPS6 Ab.](http://images.novusbio.com/fullsize2/Ribosomal-Protein-S6-RPS6-Antibody-Western-Blot-NB100-1595-img0001.jpg)
![Immunohistochemistry: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- RPS6 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize2/Ribosomal Protein S6-RPS6 Antibody-Immunohistochemistry-NB100-1595-img0006.jpg)
![Western Blot: PSMD7 Antibody [NBP2-38612] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize2/PSMD7-Antibody-Western-Blot-NBP2-38612-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PSMD7 Antibody [NBP2-38612] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize2/PSMD7 Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38612-img0004.jpg)
![Immunohistochemistry: PSMD7 Antibody [NBP2-38612] - Staining of human hippocampus shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize2/PSMD7-Antibody-Immunohistochemistry-NBP2-38612-img0002.jpg)
![Western Blot: 5-HT1B Antibody [NB100-56350] - Analysis of 5-HT1B in human brain lysate with anti-5-HT1BR pcAb (NB100-56350). A protein band of approximate molecular weight of 40-41kDa was detected.](http://images.novusbio.com/fullsize2/5-HT1B-Antibody-Western-Blot-NB100-56350-img0004.jpg)
![Western Blot: RPS10 Antibody [NBP1-31357] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize2/RPS10 Antibody-Western Blot-NBP1-31357-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: RPS10 Antibody [NBP1-31357] - Analysis of HeLa, using NBP1-31357 at 1:200 dilution.](http://images.novusbio.com/fullsize2/RPS10 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31357-img0003.jpg)
![Immunohistochemistry-Paraffin: RPS10 Antibody [NBP1-31357] - Paraffin-embedded mouse colon diluted at 1:500.](http://images.novusbio.com/fullsize2/RPS10-Antibody-Immunohistochemistry-Paraffin-NBP1-31357-img0008.jpg)
![Western Blot: PSMD6 Antibody [NBP1-81545] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize2/PSMD6-Antibody-Western-Blot-NBP1-81545-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: PSMD6 Antibody [NBP1-81545] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles.](http://images.novusbio.com/fullsize2/PSMD6 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81545-img0007.jpg)
![Immunohistochemistry-Paraffin: PSMD6 Antibody [NBP1-81545] - Staining of human hippocampus shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize2/PSMD6-Antibody-Immunohistochemistry-Paraffin-NBP1-81545-img0002.jpg)
![Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - RPS5 monoclonal antibody (M02), clone 4H3. Analysis of RPS5 expression in NIH/3T3.](http://images.novusbio.com/fullsize2/RPS5-Antibody-(4H3)-Western-Blot-H00006193-M02-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: RPS5 Antibody (4H3) [H00006193-M02] - Analysis of monoclonal antibody to RPS5 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize2/RPS5-Antibody-(4H3)-Immunocytochemistry-Immunofluorescence-H00006193-M02-img0008.jpg)
![Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - RPS5 monoclonal antibody (M02), clone 4H3 Analysis of RPS5 expression in HeLa.](http://images.novusbio.com/fullsize2/RPS5-Antibody-(4H3)-Western-Blot-H00006193-M02-img0010.jpg)
![Western Blot: Rpn2 Antibody [NB120-2941] - Analysis of proteasome 19S subunit S1 from HeLa cell extract.](http://images.novusbio.com/fullsize2/Rpn2 Antibody-Western Blot-NB120-2941-img0003.jpg)
![Western Blot: PSMD8 Antibody [NBP1-83254] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize2/PSMD8-Antibody-Western-Blot-NBP1-83254-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PSMD8 Antibody [NBP1-83254] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize2/PSMD8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83254-img0006.jpg)
![Immunohistochemistry-Paraffin: PSMD8 Antibody [NBP1-83254] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize2/PSMD8-Antibody-Immunohistochemistry-Paraffin-NBP1-83254-img0008.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")