L1CAM Antibody (L1CAM/9146R)

Immunohistochemistry-Paraffin: L1CAM Antibody (L1CAM/9146R) [NBP3-23681] - Formalin-fixed, paraffin-embedded human brain stained with L1CAM Antibody (L1CAM/9146R). Inset: PBS instead of primary antibody; secondary only negative control.

Western Blot Analysis of Human Brain lysate using L1CAM Antibody (L1CAM/9146R)

• Reactivity: Hu
• Applications: IHC
• Clone: L1CAM/9146R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

L1CAM Antibody (L1CAM/9146R) Summary

• Description: Store at 2 to 8C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant full-length human L1CAM protein
• Localization: Cytoplasm. Cell Surface.
• Specificity: This mouse monoclonal antibody recognizes the sequence immediately NH2-terminal to the tyrosine-based motif and binds L1 only when Y1176 is dephosphorylated. Phosphorylation of either T1172 or Y1176 strongly inhibits binding of 74-5H7 to the L1 cytoplasmic domain.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: L1CAM
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2ug/ml
• Application Notes: Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for L1CAM Antibody (L1CAM/9146R)

• antigen identified by monoclonal R1
• CAML1
• CAML1N-CAML1
• CD171 antigen
• CD171
• HSAS
• HSAS1
• L1 cell adhesion molecule
• L1CAM
• MASA
• MIC5
• N-CAM-L1
• NCAM-L1
• neural cell adhesion molecule L1
• S10
• SPG1

Background

The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, adductus thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: L1CAM