![Western Blot: KMT2D Antibody [NBP3-18671] - Figure 2. Western blot analysis using the antibody directed against KMT2D (1) Western blot was performed on whole cell lysates from mouse embryonic stem cells (E14Tg2a) with the antibody against mouse KMT2D (ca. No. C15310100), diluted 1:500 in PBS-Tween containing 5% skimmed milk. The location of the protein of interest (~ 600 kDa) is indicated on the right; the location of the 500 kDa MW marker is shown on the left. Cells homozygous for the targeted mll4 allele (-/-) show a complete loss of KMT2D protein, whereas the KMT2D protein was detected in flp-recombined ES cells (F/F) and heterozygously targeted ES cells (+/-).](http://images.novusbio.com/fullsize/KMT2D-Antibody-Western-Blot-NBP3-18671-img0001.jpg "Western Blot: KMT2D Antibody [NBP3-18671] - Figure 2. Western blot analysis using the antibody directed against KMT2D (1) Western blot was performed on whole cell lysates from mouse embryonic stem cells (E14Tg2a) with the antibody against mouse KMT2D (ca. No. C15310100), diluted 1:500 in PBS-Tween containing 5% skimmed milk. The location of the protein of interest (~ 600 kDa) is indicated on the right; the location of the 500 kDa MW marker is shown on the left. Cells homozygous for the targeted mll4 allele (-/-) show a complete loss of KMT2D protein, whereas the KMT2D protein was detected in flp-recombined ES cells (F/F) and heterozygously targeted ES cells (+/-).")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Polyclonal antibody raised in rabbit against mouse KMT2D (Myeloid/lymphoid or mixed-lineage leukemia protein 4), using three KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein (1). |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | KMT2D |
| Purity | Whole Antiserum |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | Whole antiserum |
| Preservative | 0.05% Sodium Azide |
| Purity | Whole Antiserum |
Secondary Antibodies |
Isotype Controls |
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![ELISA: KMT2D Antibody [NBP3-18671] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse KMT2D . The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:54,000.](http://images.novusbio.com/fullsize/KMT2D Antibody-ELISA-NBP3-18671-img0002.jpg "ELISA: KMT2D Antibody [NBP3-18671] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse KMT2D . The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:54,000.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
