Mouse Klotho ELISA Kit (Colorimetric)

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ELISA: Mouse Klotho ELISA Kit (Colorimetric) [NBP3-18030] - Samples were spiked with high concentrations of Mouse Klotho and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Mouse Klotho ELISA Kit (Colorimetric) [NBP3-18030] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
15.63-1000 pg/mL
Sensitivity
9.38 pg/mL

Order Details

Mouse Klotho ELISA Kit (Colorimetric) Summary

Standard Curve Range
15.63-1000 pg/mL
Sensitivity
9.38 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 5.61
Intra-Assay
%CV < 5.19
Spike Recovery
87-100%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
KL

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse Klotho ELISA Kit (Colorimetric)

  • EC 3.2.1
  • EC 3.2.1.31
  • KL
  • Klotho

Background

The Klotho gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Klotho ELISA Kit (NBP3-18030). (Showing 1 - 2 of 2 FAQ).

  1. I study physiology (PhD) and I need a klotho kit.
    • A list of our klotho products can be found here. I am not entirely sure what you are looking for as I don't have your complete message for some reason. Can you please clarify what you are looking for?
  2. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol KL