KLHL24 Antibody (818235) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: Flow
• Clone: 818235
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

KLHL24 Antibody (818235) [Alexa Fluor® 488] Summary

• Immunogen: Chinese Hamster Ovary cell line CHO-derived human GluR6/GRIK2 protein
  Thr32-Asn549
  Accession # Q13002
• Specificity: Detects human GluR6/GRIK2 in cell-based ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KLHL24 Antibody (818235) [Alexa Fluor® 488]

• DRE1
• EAA4
• GLR6
• GluK2
• GLUK6
• GluR6
• Kainate Receptor Interacting Protein For GluR6
• Kainate Receptor-Interacting Protein For GluR6
• Kelch-Like 24 (Drosophila)
• Kelch-Like 24
• Kelch-Like Family Member 24
• Kelch-Like Protein 24
• KLHL24
• KRIP6
• MRT6
• Protein DRE1

Background

The Ionotropic Glutamate Receptors are a family of ligand-gated ion channels involved in regulating fast excitatory synaptic transmission in the central nervous system (CNS) (1, 2). The family consists of ~18 members which is divided into 3 groups based on the activating agonist: N-Methyl-D-Aspartate (NMDA), alpha -Amino-3-hydroxy-5-Methyl-4-isoxazole Propionic Acid (AMPA) and kainate (KA) (1, 2). KA receptors (KAR) are found in the pre- and postsynaptic membranes of the central nervous system, most prominently in the hippocampus, where they regulate synaptic transmission, neuronal excitability, and network activity (3). Glutamate receptor 6 (GluR6) (also Glutamate receptor ionotropic, kainate 2 or GluK2) along with GluR5, GluR7 and receptor subunits KA1 and KA2 belong to the KAR sub-group as these molecules are activated by kainic acid (1-3). Mature human GluR6 is a 3-transmembrane protein with a long (530 aa) N-terminal extracellular domain (ECD), a M2 domain involved in pore formation and a short C-terminal intracellular domain (4). The ECD of human GluR6 is highly conserved and shares 99% identity with both mouse and rat. Under physiological conditions, GluR6 forms either a homomeric or heteromeric receptor channel with GluR5 or KA1/2 to become functional (5, 6). Activation of KAR complexes result in Ca2⁺-dependent release of glutamatergic or GABAergic signals (7). The modulatory role of KARs make them attractive therapeutic candidates as glutamate excitotoxicity has been associated with multiple neurodegenerative and mental disorders. Misregulation of GluR6 has been suggested to be a factor in autism, Huntington disease and epilepsy (8). Additionally, GluR6 has been suggested to play a role in tumor suppression in Gastric cancer (9) while potentially displaying the opposite effect in urological cancers (10).

1. Dingledine R et al. (1999) Pharmacol Rev. 51(1):7.
2. Traynelis S.F et al. (2010) Pharmacol Rev. 62(3):405.
3. Contractor A et al. (2011) Trends Neurosci. 34(3):154.
4. Nanao MH et al. (2005) PNAS 102(5):1708.
5. Kumar J et al. (2011) Neuron. 71(2):319.
6. Fisher JL et al. (2011) J Neurosci. 31(47):17113.
7. Mathew SS and Hablitz JJ (2008) Neuropharmacology. 55(1):106.
8. Lerma J and Marques JM (2013) Neuron. 80(2):292.
9. Wu CS et al. (2010) Int J Cancer. 126(11):2542.
10. Inoue R. et al. (2017) Oncotarget. 8(17):28826.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q13002(Human)