KLF2 Antibody (OTI3A10) - Azide and BSA Free

Western Blot: KLF2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71597] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLF2.

Immunohistochemistry: KLF2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71597] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min)

Immunohistochemistry: KLF2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71597] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI3A10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

KLF2 Antibody (OTI3A10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse KLF2 Antibody (OTI3A10) - Azide and BSA Free (NBP2-45510) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human KLF2 (NP_057354) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 37.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for KLF2 Antibody (OTI3A10) - Azide and BSA Free

• KLF2
• Krueppel-like factor 2
• Kruppel-like factor 2 (lung)
• Kruppel-like factor LKLF
• LKLF
• LKLFLung krueppel-like factor
• lung Kruppel-like zinc finger transcription factor

Background

KLF2 binds to the CACCC box in the beta-globin gene promoter and activates transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLF2