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KLF2 Antibody (OTI3A10) [DyLight 488]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
OTI3A10
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71597AF350
Alexa Fluor 405
NBP2-71597AF405
Alexa Fluor 488
NBP2-71597AF488
Alexa Fluor 532
NBP2-71597AF532
Alexa Fluor 594
NBP2-71597AF594
Alexa Fluor 647
NBP2-71597AF647
Alexa Fluor 700
NBP2-71597AF700
Alexa Fluor 750
NBP2-71597AF750
Biotin
NBP2-71597B
DyLight 350
NBP2-71597UV
DyLight 405
NBP2-71597V
DyLight 550
NBP2-71597R
DyLight 594
NBP2-71597DL594
DyLight 650
NBP2-71597C
DyLight 680
NBP2-71597FR
DyLight 755
NBP2-71597IR
FITC
NBP2-71597F
HRP
NBP2-71597H
Janelia Fluor 549
NBP2-71597JF549
Janelia Fluor 646
NBP2-71597JF646
Unconjugated
NBP2-45510
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45510
Azide and BSA Free
NBP2-71597
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KLF2 Antibody (OTI3A10) [DyLight 488] Summary

Immunogen
Full length recombinant protein of human KLF2 (NP_057354) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KLF2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for KLF2 Antibody (OTI3A10) [DyLight 488]

  • KLF2
  • Krueppel-like factor 2
  • Kruppel-like factor 2 (lung)
  • Kruppel-like factor LKLF
  • LKLF
  • LKLFLung krueppel-like factor
  • lung Kruppel-like zinc finger transcription factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-24749
Immunohistochemistry: KLF4 Antibody [NBP2-24749] - DAB IHC staining. Representative 3,3-diaminobenzidine immunohistochemical-stained images showing protein expression of induced-pluripotent stem cell marker KLF4 (J-L, brown) in normal colon (J), low-grade (K) and high-grade (L) colon adenocarcinoma tissue samples. Nuclei were counter-stained with hematoxylin (A-O, blue). Original magnification: 400x. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0221963) licensed under a CC-BY licence.
Western Blot: KLF4 Antibody [NBP2-24749] - Total protein from Human PC3, Hek293 and Mouse Neuro2A cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-KLF4 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunohistochemistry: KLF4 Antibody [NBP2-24749] - IF IHC staining. Representative immunofluorescence immunohistochemical-stained images showing protein expression of induced-pluripotent stem cell markers OCT4 (A-C, green), KLF4 (A-C, red) in normal colon (A), low-grade (B) and high-grade (C) colon adenocarcinoma tissue samples. Cell nuclei were counter-stained with 4', 6'-diamidino-2-phenylindole (A-C, blue). Original magnification: 400x. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0221963) licensed under a CC-BY licence.
(1 Review)
(19 Publications)
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(32 Reviews)
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eNOS Antibody
NB300-500
Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Expression of endothelial markers indicate functional endothelium in TEVG. Whole mount staining of native aorta and TEVG. VE-cadherin is a marker of cellular borders of endothelial cells (green). Endothelial nitric oxide synthase (eNOS) is a marker of a functional endothelium (red). DAPI is a nuclear stain (blue). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0120328), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Staining of eNOS in human lung tissue.
Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Mouse Carotid Artery stained with eNOS antibody. Image from verified customer review.
(3 Reviews)
(17 Publications)
Species: Hu, Mu, Rt, Po, Am, Bv, Ca, Dr, Sh
Applications: WB, IHC, IHC-Fr, IHC-P
KLF1 Antibody
(1B6A3)
NBP2-37380
Western Blot: KLF1 Antibody (1B6A3) [NBP2-37380] - Western blot analysis using KLF1 mAb against human KLF1 recombinant protein. (Expected MW is 42.6 kDa)
Immunohistochemistry: KLF1 Antibody (1B6A3) [NBP2-37380] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using KLF1 mouse mAb with DAB staining.
Flow Cytometry: KLF1 Antibody (1B6A3) [NBP2-37380] - Flow cytometric analysis of HeLa cells using KLF1 mouse mAb (green) and negative control (red).
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
ERK5/BMK1 Antibody
[Unconjugated]
AF2848
Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Neuro‑2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ERK5/BMK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2848) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ERK5/BMK1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ERK5/BMK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse ERK5/BMK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2848) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Review)
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Applications: WB, ICC
L-Selectin/CD62L
728-LS
L-Selectin/CD62L
(10 Publications)
Species: Hu

Thrombomodulin/BDCA-3 ...
AF3894
Thrombomodulin/BDCA‑3 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse Thrombomodulin/BDCA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3894) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Thrombomodulin/BDCA‑3 was detected in immersion fixed paraffin-embedded sections of human kidney tissue using Goat Anti-Mouse Thrombomodulin/BDCA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3894) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface on endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Species: Mu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ICC
KLF5 Antibody
[Unconjugated]
AF3758
Western blot shows lysates of BG01V human embryonic stem cells, D3 mouse embryonic stem cell line, and P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for KLF5 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of D3 mouse embryonic stem cell line, loaded at 0.2 mg/mL. A specific band was detected for KLF5 at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and KLF5 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for KLF5 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockoutHeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Nrf2 Antibody
NBP1-32822
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NFkB p105/p50
Antibody (2J10D7)
NB100-56583
Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.
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Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.
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c-jun Antibody
(5B1)
NBP2-37576
Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human rectum cancer tissues using c-Jun mouse mAb with DAB staining.
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
OCT4 Antibody
NB100-2379
Western Blot: OCT4 Antibody [NB100-2379] - YM155 significantly reduced tumorsphere formation and inhibited EGFR autophosphorylation and G9a expression. YM155 is reported to suppress cancer stemness; therefore, we used this compound to investigate the cellular mechanism of tumorsphere formation. To compare the cytotoxic capacity of YM155 against tumorsphere formation and parental cell lines, YM155 was applied to HCC827 and A549 cells in the stemness cultured or integrated medium. (F) Moreover, YM155 inhibited EGFR autophosphorylation in A549-derived tumorspheres and reduced Oct4 expression. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0182149) licensed under a CC-BY licence.
Western Blot: OCT4 Antibody [NB100-2379] - WB detection of OCT4 in mouse brain lysate using OCT4 antibody with ECL based detection. The antibody generated a specific band of OCT4 at the expected ~40kDa position.
Flow Cytometry: OCT4 Antibody [NB100-2379] - An intracellular stain was performed on Jurkat with NB100-2379 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.
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Recombinant Human
MEK5 GST (N-Term) Pro ...
H00005607-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
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MEF2A Antibody
(JA33-04)
NBP2-66943
Western Blot: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of MEF2A on rat spleen tissue lysate using anti-MEF2A antibody at 1/1,000 dilution.
Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded mouse spleen tissue using anti-MEF2A antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded human spleen tissue using anti-MEF2A antibody. Counter stained with hematoxylin.
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SP1 Antibody
NB600-233
Western Blot: SP1 Antibody [NB600-233] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Chromatin Immunoprecipitation: SP1 Antibody [NB600-233] - Chromatin from acute myeloid leukemia cell line was immunoprecipitated with anti-Sp1 antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of Sp1 binding within a 250 Kb region of chromosome 6 asdetected using anti-Sp1.
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Applications: WB, ChIP, IHC, IHC-P, IP, CHIP-SEQ
Endothelin-1 [HRP]
QET00B
(3 Reviews)
(35 Publications)
Species: Hu
COCO/DAND5
Antibody (OTI2H3)
NBP2-01223
Western Blot: DAND5 Antibody (2H3) [NBP2-01223] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAND5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAND5.
Immunohistochemistry-Paraffin: DAND5 Antibody (2H3) [NBP2-01223] Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DAND5 mouse monoclonal antibody.
Flow Cytometry: DAND5 Antibody (2H3) [NBP2-01223] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DAND5 antibody, and then analyzed by flow cytometry.
Species: Hu
Applications: WB, Flow, IHC, IHC-P
c-Myc Antibody
NB600-335
Western Blot: c-Myc Antibody [NB600-335] - Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-c-myc antibody NB600-335 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-335] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-c-Myc conjugated to Alexa Fluor 488 [NB600-335AF488] at 20ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the Alexa Fluor 488 format of this antibody.
Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ug/ml (1:25,000).
(1 Review)
(49 Publications)
Species: Hu, Mu, Ec, All-NA
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-71597AF350
Alexa Fluor 405 NBP2-71597AF405
Alexa Fluor 488 NBP2-71597AF488
Alexa Fluor 532 NBP2-71597AF532
Alexa Fluor 594 NBP2-71597AF594
Alexa Fluor 647 NBP2-71597AF647
Alexa Fluor 700 NBP2-71597AF700
Alexa Fluor 750 NBP2-71597AF750
Biotin NBP2-71597B
DyLight 350 NBP2-71597UV
DyLight 405 NBP2-71597V
DyLight 488 NBP2-71597G
DyLight 550 NBP2-71597R
DyLight 594 NBP2-71597DL594
DyLight 650 NBP2-71597C
DyLight 680 NBP2-71597FR
DyLight 755 NBP2-71597IR
FITC NBP2-71597F
HRP NBP2-71597H
Janelia Fluor 549 NBP2-71597JF549
Janelia Fluor 646 NBP2-71597JF646
Learn about Custom Antibody Labeling

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Bioinformatics Tool for KLF2 Antibody (NBP2-71597G)

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Blogs on KLF2.

Check out the latest blog posts on KLF2.
KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.
Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.
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Bioinformatics

Gene Symbol KLF2