KLF12 Antibody (PCRP-KLF12-1E3)

Immunocytochemistry/Immunofluorescence: KLF12 Antibody (PCRP-KLF12-1E3) [NBP3-13848] - Immunofluorescence analysis of PFA-fixed HeLa cells. KLF12 Antibody (PCRP-KLF12-1E3) followed by goat anti-mouse IgG-CF488 (green).

Flow Cytometry: KLF12 Antibody (PCRP-KLF12-1E3) [NBP3-13848] - Flow cytometric analysis of PFA-fixed HeLa cells. KLF12 antibody (PCRP-KLF12-1E3) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

Protein Array: KLF12 Antibody (PCRP-KLF12-1E3) [NBP3-13848] - Analysis of Protein Array containing more than 19,000 full-length human proteins using KLF12 Antibody (PCRP-KLF12-1E3).

• Reactivity: Hu
• Applications: Flow, ICC/IF, IP, MA
• Clone: PCRP-KLF12-1E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

KLF12 Antibody (PCRP-KLF12-1E3) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14277)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human KLF12 protein (Uniprot: Q9Y4X4)
• Localization: Nucleus
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLF12
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
• Theoretical MW: 44.24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react with Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for KLF12 Antibody (PCRP-KLF12-1E3)

• AP-2rep transcription factor
• AP2REP
• AP-2rep
• AP2REPAP-2 repressor
• HSPC122
• KLF12 zinc finger transcriptional repressor
• KLF12
• Krueppel-like factor 12
• Kruppel-like factor 12
• Transcriptional repressor AP-2rep

Background

KLF12 is a gene the codes for a protein that bonds to A32 in the AP-2-alpha gene promoter in order to facilitate the transcriptional repression to the AP-2-alpha gene. KLF12 has two isoforms, with lengths of 402 and 269 amino acids, and weights of approximately 44 and 28 kDa respectively. Current studies are being done on diseases and disorders relating to this gene, including Wegener's granulomatosis, rheumatoid arthritis, gastric cancer, pancreatic cancer, pancreatitis, and schizophrenia. KLF12 has also been shown to have interactions with EHMT2, CTBP1, and SIRT2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLF12
• Uniprot:
  • Q9Y4X4()
  • Q9Y4X4()